Western Blot (WB)
(RNASE1 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RNASE1 antibody detected the RNASE1 protein (arrow).)
Rabbit anti-Human RNASE1 Polyclonal Antibody | anti-RNASE1 antibody
RNASE1, NT (RNASE1, RIB1, RNS1, Ribonuclease pancreatic, HP-RNase, RIB-1, RNase UpI-1, Ribonuclease 1, Ribonuclease A) (FITC)
Gene Names
RNASE1; RIB1; RNS1
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RNASE1; Polyclonal Antibody; NT (RNASE1; RIB1; RNS1; Ribonuclease pancreatic; HP-RNase; RIB-1; RNase UpI-1; Ribonuclease 1; Ribonuclease A) (FITC); Ribonuclease A; anti-RNASE1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-RNASE1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
RNASE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-42 amino acids from the N-terminal region of human RNASE1.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(RNASE1 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RNASE1 antibody detected the RNASE1 protein (arrow).)
Western Blot (WB)
(RNASE1 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RNASE1 antibody detected the RNASE1 protein (arrow).)
Related Product Information for anti-RNASE1 antibody
This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq].
Product Categories/Family for anti-RNASE1 antibody
References
D'Alessio, G. Biopolymers 91(12):989-994(2009). Kover, K.E., et al. J. Mol. Biol. 379(5):953-965(2008). Ueki, M., et al. Biochem. Genet. 46 (3-4), 145-153 (2008): Johnson, R.J., et al. Biochemistry 46(36):10308-10316(2007). Cybulski, C., et al. Urol. Int. 79(1):44-49(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,644 Da
NCBI Official Full Name
ribonuclease pancreatic
NCBI Official Synonym Full Names
ribonuclease, RNase A family, 1 (pancreatic)
NCBI Official Symbol
RNASE1
NCBI Official Synonym Symbols
RIB1; RNS1
NCBI Protein Information
ribonuclease pancreatic; RIB-1; RNase 1; RNase A; HP-RNase; RNase upI-1; ribonuclease 1; ribonuclease A
UniProt Protein Name
Ribonuclease pancreatic
Protein Family
UniProt Gene Name
RNASE1
UniProt Synonym Gene Names
RIB1; RNS1; RNase 1; RNase A
NCBI Description
This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA.
Research Articles on RNASE1
Similar Products
Product Notes
The RNASE1 rnase1 (Catalog #AAA6342382) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNASE1, NT (RNASE1, RIB1, RNS1, Ribonuclease pancreatic, HP-RNase, RIB-1, RNase UpI-1, Ribonuclease 1, Ribonuclease A) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNASE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RNASE1 rnase1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNASE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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