Ribonuclease A (RNASE1) Recombinant Protein | RNASE1 recombinant protein

Recombinant Ribonuclease A (RNASE1)

Cat. Num. AAA2009838

• Gene Names: RNASE1; RNS1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Ribonuclease A (RNASE1); Recombinant Ribonuclease A (RNASE1); RNASE1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-AAKFERQHMD SSTSAASSSN YCNQMMKSRN LTKDRCKPVN TFVHESLADV QAVCSQKNVA CKNGQTNCYQ SYSTMSITDC RETGSSKYPN CAYKTTQANK HIIVACEGNP YVPVHFDASV
• Sequence Length: 150

• Applicable Applications for RNASE1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Bos taurus; Bovine (Cattle)
• Expression System: Prokaryotic expression
• Residues: Ala31~Val150 (Accession # P61823) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 68299789
• NCBI GeneID: 282340
• NCBI Accession #: NP_001014408.2
• NCBI GenBank Nucleotide #: NM_001014386.4
• UniProt Accession #: P61823; P00656; Q9TSF2; A6QPW8
• Molecular Weight: 17.1kDa
• NCBI Official Full Name: ribonuclease pancreatic
• NCBI Official Synonym Full Names: ribonuclease, RNase A family, 1 (pancreatic)<
• NCBI Official Symbol: RNASE1
• NCBI Official Synonym Symbols: RNS1
• NCBI Protein Information: ribonuclease pancreatic; RNase 1; RNase A; ribonuclease A (pancreatic)
• UniProt Protein Name: Ribonuclease pancreatic
• Protein Family: Ribonuclease pancreatic
• UniProt Gene Name: RNASE1
• UniProt Synonym Gene Names: RNS1
• UniProt Entry Name: RNAS1_BOVIN

Uniprot Description

Function: Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA. Ref.3

Catalytic activity: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Subunit structure: Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein

By similarity. Monomer.

Subcellular location: Secreted.

Tissue specificity: Pancreas.

Miscellaneous: Ribonuclease can destabilize or unwind the DNA helix by complexing with single-stranded DNA; this complex arises by an extended multisite cation-anion interaction between the lysine and arginine residues of the enzyme and the phosphate groups of the nucleotides.

Sequence similarities: Belongs to the pancreatic ribonuclease family.