Cyclin D1 (CCND1) Recombinant Protein | CCND1 recombinant protein

Recombinant Cyclin D1 (CCND1)

Cat. Num. AAA2011773

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cyclin D1 (CCND1); Recombinant Cyclin D1 (CCND1); CCND1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-KCEEEVFPL AMNYLDRFLS LEPVKKSRLQ LLGATCMFVA SKMKETIPLT AEKLCIYTDN SIRPDELLHM ELVLVNKLKW NLAAMTPHDF IEHFLSKMPV AEENKQIIRK HAQTFVALCA TDVKFISNPP SMVAAGSVAA AAQGLHLGSA NGFLSYHRLT RFLSKVIRCD PDCLRACQEQ IEALLESSLR QAQQQNLDPK AA
• Sequence Length: 295

• Applicable Applications for CCND1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Bos taurus; Bovine (Cattle)
• Expression System: Prokaryotic expression
• Residues: Lys72~Ala272 (Accession # Q2KI22) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Nucleus. Cytoplasm. Membrane
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.2
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 114053227
• NCBI GeneID: 524530
• NCBI Accession #: NP_001039738.1
• NCBI GenBank Nucleotide #: NM_001046273.2
• UniProt Accession #: Q2KI22
• Molecular Weight: 26.3kDa
• NCBI Official Full Name: G1/S-specific cyclin-D1
• NCBI Official Synonym Full Names: cyclin D1<
• NCBI Official Symbol: CCND1
• NCBI Protein Information: G1/S-specific cyclin-D1
• UniProt Protein Name: G1/S-specific cyclin-D1
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: CCND1
• UniProt Entry Name: CCND1_BOVIN

Uniprot Description

Function: Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex

By similarity.

Subunit structure: Interacts with either CDK4 or CDK6 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Component of the ternary complex cyclin D/CDK4/CDKN1B required for nuclear translocation and modulation of CDK4-mediated kinase activity. Interacts directly with CDKN1B. Can form similar complexes with either CDKN1A or CDKN2A. Interacts with USP2 and FBXO4. Interacts with UHRF2; the interaction ubiquitinates CCND1 and appears to occur independently of phosphorylation

By similarity.

Subcellular location: Nucleus

By similarity. Cytoplasm

By similarity. Membrane

By similarity. Note: Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members

By similarity.

Post-translational modification: Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex

By similarity.Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB. Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. SCF-type ubiquitination is dependent on Thr-286 phosphorylation. Ubiquitinated also by UHRF2 apparently in a phosphorylation-independent manner. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to its stabilization

By similarity.

Sequence similarities: Belongs to the cyclin family. Cyclin D subfamily.Contains 1 cyclin N-terminal domain.