Rabbit RAMP3 Polyclonal Antibody | anti-RAMP3 antibody

RAMP3 Polyclonal Antibody

Cat. Num. AAA3011688

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RAMP3; Polyclonal Antibody; RAMP3 Polyclonal Antibody; Receptor activity-modifying protein 3; Calcitonin-receptor-like receptor activity-modifying protein 3; CRLR activity-modifying protein 3; anti-RAMP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RAMP3 polyclonal antibody detects endogenous levels of RAMP3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 148

• Applicable Applications for anti-RAMP3 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human RAMP3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RAMP3 polyclonal antibody)

Related Product Information for anti-RAMP3 antibody

Receptor activity-modifying proteins (RAMPs) are transmembrane accessory proteins that influence the pharmacological profiles of the calcitonin receptor-like receptors (CRLR). RAMPs associate with CRLR in the endoplasmic reticulum and facilitate the glycosylation and transport of CRLR to the cell surface, where the mature protein then operates as a receptor for two structurally related vasodilatory peptides, calcitonin-gene-related peptide (CGRP) or adrenomedullin (ADM). RAMP-1 associating with CRLR confers a CGRP receptor, while RAMP-2 and RAMP-3 preferentially induce a responsiveness to ADM. RAMP proteins, including RAMP-1, RAMP-2 and RAMP-3, are structurally similar as they are type I receptors, which have a single extracellular N-terminus and a cytoplasmic C-terminus, and they share approximately 55% sequence similarity. RAMP-1 expression is highest in the uterus, brain and gastrointestinal tract, whereas RAMP-2 and RAMP-3 are highest in lung, breast and fetal tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 11134424
• NCBI GeneID: 10268
• UniProt Accession #: O60896
• Molecular Weight: ~ 40kDa
• NCBI Official Full Name: Receptor activity-modifying protein 3
• NCBI Official Synonym Full Names: receptor activity modifying protein 3
• NCBI Official Symbol: RAMP3
• NCBI Protein Information: receptor activity-modifying protein 3
• UniProt Protein Name: Receptor activity-modifying protein 3
• Protein Family: Receptor activity-modifying protein
• UniProt Gene Name: RAMP3
• UniProt Synonym Gene Names: CRLR activity-modifying protein 3
• UniProt Entry Name: RAMP3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP3) protein, CRLR functions as an adrenomedullin receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

RAMP3: Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL. Belongs to the RAMP family.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7p13-p12

Cellular Component: cell surface; integral to plasma membrane; lysosome; plasma membrane; intracellular; receptor complex

Molecular Function: protein binding; coreceptor activity; receptor activity; protein transporter activity

Biological Process: intracellular protein transport; protein transport; receptor internalization; calcium ion transport; negative regulation of transcription, DNA-dependent; positive regulation of receptor recycling; regulation of G-protein coupled receptor protein signaling pathway