Mouse receptor-interacting serine-threonine kinase 3 ELISA Kit | RIPK3 elisa kit

Mouse Receptor-interacting serine/threonine-protein kinase 3, RIPK3 ELISA Kit

Cat. Num. AAA930974

• Gene Names: Ripk3; Rip3; AW107945; 2610528K09Rik
• Reactivity: Mouse
• Synonyms: receptor-interacting serine-threonine kinase 3; Mouse Receptor-interacting serine/threonine-protein kinase 3; RIPK3 ELISA Kit; Mouse Receptor-interacting serine/threonine-protein kinase 3 (RIPK3) ELISA kit; RIP3; RIP-like protein kinase 3; receptor interacting protein 3; RIPK3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse RIPK3. No significant cross-reactivity or interference between Mouse RIPK3 and analogues was observed.
• Sequence Length: 486

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 46.88 pg/ml-3000 pg/ml
• Sensitivity: 11.72 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Immunity
• Protein Biological Process 3: Host-virus interaction
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RIPK3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RIPK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RIPK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RIPK3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RIPK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 256017133
• NCBI GeneID: 56532
• NCBI Accession #: NP_064339.2
• NCBI GenBank Nucleotide #: NM_019955.2
• UniProt Accession #: Q9QZL0; Q3U3Z9; Q8K2Y2; G3X8V8
• Molecular Weight: 53,322 Da
• NCBI Official Full Name: receptor-interacting serine/threonine-protein kinase 3 isoform 1
• NCBI Official Synonym Full Names: receptor-interacting serine-threonine kinase 3
• NCBI Official Symbol: Ripk3
• NCBI Official Synonym Symbols: Rip3; AW107945; 2610528K09Rik
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 3; RIP-3; mRIP3; RIP-like protein kinase 3; receptor interacting protein 3; receptor-interacting protein 3
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 3
• Protein Family: Receptor-interacting serine/threonine-protein kinase
• UniProt Gene Name: Ripk3
• UniProt Synonym Gene Names: Rip3; RIP-3; mRIP3
• UniProt Entry Name: RIPK3_MOUSE

Uniprot Description

RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, TKL; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); TKL group; RIPK family

Cellular Component: membrane; mitochondrion; cytoplasm; plasma membrane; intracellular; cytosol

Molecular Function: transferase activity; NF-kappaB-inducing kinase activity; identical protein binding; protein serine/threonine kinase activity; protein binding; transferase activity, transferring phosphorus-containing groups; protein complex binding; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: spleen development; regulation of activated T cell proliferation; I-kappaB kinase/NF-kappaB cascade; viral reproduction; thymus development; protein heterooligomerization; protein amino acid autophosphorylation; T cell differentiation in the thymus; programmed cell death; positive regulation of transferase activity; lymph node development; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; regulation of T cell mediated cytotoxicity; regulation of adaptive immune response; regulation of interferon-gamma production; activation of protein kinase activity; T cell homeostasis; positive regulation of ligase activity; phosphorylation; protein homooligomerization; positive regulation of oxidoreductase activity