Human receptor-interacting serine-threonine kinase 3 ELISA Kit | RIPK3 elisa kit

Human Receptor-interacting serine/threonine-protein kinase 3, RIPK3 ELISA Kit

Cat. Num. AAA926196

• Gene Names: RIPK3; RIP3
• Reactivity: Human
• Synonyms: receptor-interacting serine-threonine kinase 3; Human Receptor-interacting serine/threonine-protein kinase 3; RIPK3 ELISA Kit; Human Receptor-interacting serine/threonine-protein kinase 3 (RIPK3) ELISA kit; RIP3; RIP-like protein kinase 3; receptor interacting protein 3; RIPK3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human RIPK3. No significant cross-reactivity or interference between human RIPK3 and analogues was observed.
• Sequence Length: 518

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: < 3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RIPK3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RIPK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RIPK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RIPK3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RIPK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 40254844
• NCBI GeneID: 11035
• NCBI Accession #: NP_006862.2
• NCBI GenBank Nucleotide #: NM_006871.3
• UniProt Accession #: Q9Y572; Q5J795; Q5J796; Q6P5Y1; B4DJL9; C4AM87
• Molecular Weight: 56,887 Da
• NCBI Official Full Name: receptor-interacting serine/threonine-protein kinase 3
• NCBI Official Synonym Full Names: receptor-interacting serine-threonine kinase 3
• NCBI Official Symbol: RIPK3
• NCBI Official Synonym Symbols: RIP3
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3; receptor interacting protein 3; receptor-interacting protein 3
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 3
• Protein Family: Receptor-interacting serine/threonine-protein kinase
• UniProt Gene Name: RIPK3
• UniProt Synonym Gene Names: RIP3; RIP-3
• UniProt Entry Name: RIPK3_HUMAN

NCBI Description

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008]

Uniprot Description

RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; RIPK family

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: mitochondrion; plasma membrane; cytosol

Molecular Function: NF-kappaB-inducing kinase activity; identical protein binding; protein serine/threonine kinase activity; protein binding; transcription coactivator activity; protein complex binding; ATP binding; protein kinase activity

Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; regulation of activated T cell proliferation; thymus development; protein heterooligomerization; protein amino acid autophosphorylation; MyD88-independent toll-like receptor signaling pathway; T cell differentiation in the thymus; protein modification process; toll-like receptor 3 signaling pathway; signal transduction; lymph node development; activation of NF-kappaB transcription factor; regulation of T cell mediated cytotoxicity; regulation of adaptive immune response; regulation of interferon-gamma production; toll-like receptor signaling pathway; positive regulation of interferon type I production; activation of protein kinase activity; innate immune response; T cell homeostasis; positive regulation of ligase activity; toll-like receptor 4 signaling pathway; protein homooligomerization; positive regulation of oxidoreductase activity