Interleukin-17 A/F Active Protein | rIL 17 A/F active protein

Recombinant Rat Interleukin-17 A/F Heterodimer

Cat. Num. AAA143518

• Gene Names: IL17A; IL17; CTLA8; IL-17; IL-17A
• Purity: Greater than 98.0% as determined by HPLC and SDS-PAGE.
• Synonyms: Interleukin-17 A/F; Recombinant Rat Interleukin-17 A/F Heterodimer; IL 17A/F Rat; Interleukin-17A/F Heterodimer Rat Recombinant; IL17A/F; IL17 A/F; IL-17A/F; IL-17 A/F; IL17AF; IL-17 AF; Interleukin-17 AF; rIL 17 A/F active protein

• Purity/Purification: Greater than 98.0% as determined by HPLC and SDS-PAGE.
• Form/Format: Lyophilized from a concentrated (1mg/ml) solution containing no additives.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: IL-17A: MAVLIPQSSV CPNAEANNFL QNVKVNLKVL NSLSSKASSR RPSDYLNRST SPWTLSRNED PDRYPSVIWE AQCRHQRCVN AEGKLDHHMN SVLIQQEILV LKREPEKCPF TFRVEKMLVG VGCTCVSSIV RHASIL-17F: MARRNPKVGL SALQKAGNCP PLEDNSVRVD IRIFNQNQGI SVPRDFQNRS SSPWDYNITR DPDRFPSEIA EAQCRHSGCI NAQGQEDGSM NSVPIQQEIL VLRREPQGCS NSFRLEKMLI KVGCTCVTPI VHHAA

• Source: E Coli
• Solubility: It is recommended to reconstitute the lyophilized Rat IL-17A/F in sterile water not more than 1mg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED50 as determined by its ability to induce of IL-6 production from NIH-3T3 cells, is 15.3-23 ng/ml.
• Preparation and Storage: Lyophilized Rat IL-17A/F although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Rat IL-17A/F should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for rIL 17 A/F active protein

Description: IL-17A/F Rat Recombinant produced in E Coli is a heterodimeric, non-glycosylated polypeptide chain containing 1 monomeric subunit of each IL-17A & IL-17F. The dimer contains 269 amino acids and having a total molecular mass of 30.7 kDa. The IL-17A/F Rat is purified by proprietary chromatographic techniques.

Introduction: Rat IL-17A/F is a glycoprotein which is secreted as a disulfide-linked heterodimer. IL-17A/F consists of two proteins of the IL-17 family, IL-17A and IL17F. Proteins of the 6 homodimeric IL17 family show a cysteine knot motif that contains two disulfide-bonds. Rat IL17A is produced as a 155 a.a precursor that includes a 23 amino acids signal sequence and a 132 amino acid chain that includes an N-linked glycosylation site. Rat IL17F is produced as a 153 amino acid precursor with a 20 amino acid signal sequence and a 133 amino acid region. Similar to IL17A, IL17F also has an N-linked glycosylation site. Both proteins (IL17A & IL17F) share 50% amino acid sequence identity. Human IL17A & IL17F show approximately 60% homology in their amino acid sequence to mouse IL-17A and IL-17F. Interleukin-17A/F and IL17A, IL17F homodimers are manufactured by activted CD4+ T cells, called Th17. IL-23 causes Th17 lymphocytes to manufacture IL-17A/F. IL17RA and IL17RC form a heterodimer for the binding of IL17A and IL17F. IL-17A/F binds IL-17RA. Interleukin-17A/F induces chemokine production and airway neutrophilia with intermediate potency between IL17A (most potent) and IL17F (least potent).

Product Categories/Family for rIL 17 A/F active protein

Cytokines and Growth Factors; Cytokines; Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 299832923
• NCBI GeneID: 3605
• Molecular Weight: 17,504 Da
• NCBI Official Full Name: interleukin 17A, partial
• NCBI Official Synonym Full Names: interleukin 17A
• NCBI Official Symbol: IL17A
• NCBI Official Synonym Symbols: IL17; CTLA8; IL-17; IL-17A
• NCBI Protein Information: interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• UniProt Protein Name: Interleukin-17A
• UniProt Gene Name: IL17A
• UniProt Synonym Gene Names: CTLA8; IL17; IL-17; IL-17A; CTLA-8
• UniProt Entry Name: IL17_HUMAN

NCBI Description

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: cytokine activity

Biological Process: cell death; cell-cell signaling; positive regulation of osteoclast differentiation; positive regulation of interleukin-23 production; apoptosis; protein amino acid glycosylation; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response