Typical Testing Data/Standard Curve (for reference only)
Interleukin-17A ELISA Kit | IL-17A elisa kit
Interleukin-17A (IL-17A)
Gene Names
IL17A; IL17; CTLA8; IL-17; IL-17A
Synonyms
Interleukin-17A; Interleukin-17A (IL-17A); IL-17A elisa kit
Sequence Length
155
Samples
Cell Culture Supernatant and mouse serum
Assay Type
Sandwich
Sensitivity
The minimum detectable dose of this mouse IL-17A ELISA kit was determined by adding two standard deviations to the mean optical density value of 20 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of mouse IL-17A calculated from calibrate I and calibrate II diluted standard curve was <2 pg/mL.
Intra-assay Precision
To determine within-run precision in Calibrator II, three different samples of known concentration were assayed by replicates of eight in one assay.
Inter-assay Precision
To determine between-run precision in Calibrator II, three different samples of known concentration were assayed by replicates on 6 different assays.
Intended Uses
This mouse Interleukin-17A ELISA Kit is designed for the in vitro quantitative determination of mouse interleukin-17A (IL-17A) concentrations in cell culture supernatant and mouse serum. This kit is intended for LABORATORY RESEARCH ONLY.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-17A elisa kit
Principle of the assay: This mouse IL-17A enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to mouse IL-17A. Standards or samples are then added to the appropriate microtiter plate wells. A biotin-conjugated antibody preparation specific for mouse IL-17A was added and incubated. Mouse IL-17A, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse IL-17A and other components in the sample. Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin, and a TMB (3,3'5, 5' tetramethyl- benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain mouse IL- 17A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of mouse IL-17A in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus mouse IL-17A concentration (pg/mL). The concentration of IL-17A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Background: Mouse Interleukin -17A (mIL-17A), also know as CTLA-8, is a secreted, homodimeric glycoprotein linked by disulfide link with a molecular mass of about 35 KD. The cytokine is the proto-type of a newly discovered pro-inflammatory cytokine family which consists of IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (IL-25) and IL-17F. IL-17A is mainly produced by a group of CD4 T helper cells termed Th17 cells. In addition to Th17 cells, CD8 T helper cells, natural killer cells and granulocytes, can also secret the cytokine after proper stimulation. IL17A exhibits structural homology and cross-species bioactivity between human and mouse cells. IL-17A acts through its receptor IL-17R. It was found to stimulate the expression of proinflammatory cytokines including IL-6, MCP-1, G-CSF, GM-CSF, IL-1alpha and TNF-alpha. Most of those cytokines plays a role in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been found to implicate with autoimmune diseases, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer, vascular inflammation and development of atherosis.
Background: Mouse Interleukin -17A (mIL-17A), also know as CTLA-8, is a secreted, homodimeric glycoprotein linked by disulfide link with a molecular mass of about 35 KD. The cytokine is the proto-type of a newly discovered pro-inflammatory cytokine family which consists of IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (IL-25) and IL-17F. IL-17A is mainly produced by a group of CD4 T helper cells termed Th17 cells. In addition to Th17 cells, CD8 T helper cells, natural killer cells and granulocytes, can also secret the cytokine after proper stimulation. IL17A exhibits structural homology and cross-species bioactivity between human and mouse cells. IL-17A acts through its receptor IL-17R. It was found to stimulate the expression of proinflammatory cytokines including IL-6, MCP-1, G-CSF, GM-CSF, IL-1alpha and TNF-alpha. Most of those cytokines plays a role in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been found to implicate with autoimmune diseases, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer, vascular inflammation and development of atherosis.
Product Categories/Family for IL-17A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,504 Da
NCBI Official Full Name
interleukin-17A
NCBI Official Synonym Full Names
interleukin 17A
NCBI Official Symbol
IL17A
NCBI Official Synonym Symbols
IL17; CTLA8; IL-17; IL-17A
NCBI Protein Information
interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
UniProt Protein Name
Interleukin-17A
Protein Family
UniProt Gene Name
IL17A
UniProt Entry Name
IL17_HUMAN
NCBI Description
The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]
Uniprot Description
IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 6p12
Cellular Component: extracellular space; external side of plasma membrane
Molecular Function: cytokine activity
Biological Process: cell death; cell-cell signaling; apoptosis; positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; protein amino acid glycosylation; immune response; positive regulation of transcription from RNA polymerase II promoter; inflammatory response
Research Articles on IL-17A
Similar Products
Product Notes
The IL-17A il17a (Catalog #AAA590054) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Interleukin-17A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
