Rabbit anti-Human, Mouse SCYL2 Polyclonal Antibody | anti-SCYL2 antibody

SCYL2, CT (SCY1-like Protein 2, Coated Vesicle-associated Kinase of 104kD, CVAK104, KIAA1360) (Biotin)

Cat. Num. AAA6498639

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: SCYL2; Polyclonal Antibody; CT (SCY1-like Protein 2; Coated Vesicle-associated Kinase of 104kD; CVAK104; KIAA1360) (Biotin); anti-SCYL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SCYL2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 929

• Applicable Applications for anti-SCYL2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human SCYL2 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-SCYL2 Pab (AP7024b) in T47D (left) and mouse brain (right) tissue lysates (35ug/lane). SCYL2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with SCYL2 (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-SCYL2 antibody

Antibodies; Protein Kinases

References

1. Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 47604944
• NCBI Accession #: NP_060458
• NCBI GenBank Nucleotide #: NP_060458.3
• UniProt Accession #: Q6P3W7
• Molecular Weight: 103.709
• NCBI Official Full Name: SCY1-like protein 2 isoform 1
• UniProt Protein Name: SCY1-like protein 2
• Protein Family: SCY1-like protein
• UniProt Gene Name: SCYL2
• UniProt Synonym Gene Names: CVAK104; KIAA1360

Uniprot Description

SCYL2: Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L- lysine. According to PubMed:16914521, has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system. Belongs to the protein kinase superfamily.

Protein type: Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); SCY1 family

Chromosomal Location of Human Ortholog: 12q23.1

Molecular Function: protein binding; receptor binding

Biological Process: endosome to lysosome transport; positive regulation of receptor internalization