RhoD recombinant protein

RhoD (Mature form), Active

Cat. Num. AAA515653

• Gene Names: RHOD; Rho; ARHD; RHOM; RHOHP1
• Applications: Western Blot
• Synonyms: RhoD; RhoD (Mature form); Active; RhoD recombinant protein

• Host: E Coli
• Form/Format: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Sequence Positions: 1-207
• Sequence Length: 1111

• Applicable Applications for RhoD recombinant protein: Enzyme Assay, Western Blot (WB)
• Species: Human
• Tag: HIS tag
• Preparation and Storage: Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Testing Data #2

Related Product Information for RhoD recombinant protein

Description: Recombinant human RhoD (1-207) was expressed in E Coli cells using an N-terminal His tag.
Scientific Background: RhoD is a member of the Rho gene family that interacts with protein kinases and serves as a target of activated GTPase. Rho binds GTP and plays a critical role in muscle differentiation by regulating the expression of myogenin and MEF2 genes. Furthermore, Rho is involved in endosome dynamics and reorganization of the actin cytoskeleton and plays a role in coordinating membrane transport (1). mDia and ROCK are downstream effectors of Rho mediating Rho action on the actin cytoskeleton; mDia produces actin filaments by nucleation and polymerization and ROCK activate myosin to cross-link them for induction of actomyosin bundles and contractility (2).

Product Categories/Family for RhoD recombinant protein

Enzymes; Active GTPases/ATPases

References

1. Gasman, S. et al: RhoD regulates endosome dynamics through Diaphanous-related Formin and Src tyrosine kinase. Nature Cell Biol. 5: 195-204, 2003. 2. Narumiya, S. et al: Rho signaling, ROCK and mDia1, in transformation, metastasis and invasion. Cancer Metastasis Rev. 2009 Jun;28 (1-2):65-76.

NCBI and Uniprot Product Information

• NCBI GI #: 12654980
• NCBI GeneID: 29984
• Molecular Weight: ~23 kDa
• NCBI Official Full Name: Homo sapiens ras homolog gene family, member D, mRNA
• NCBI Official Synonym Full Names: ras homolog family member D
• NCBI Official Symbol: RHOD
• NCBI Official Synonym Symbols: Rho; ARHD; RHOM; RHOHP1
• NCBI Protein Information: rho-related GTP-binding protein RhoD
• UniProt Protein Name: Rho-related GTP-binding protein RhoD
• Protein Family: Rhodopsin
• UniProt Gene Name: RHOD
• UniProt Synonym Gene Names: ARHD; RhoHP1

NCBI Description

Ras homolog, or Rho, proteins interact with protein kinases and may serve as targets for activated GTPase. They play a critical role in muscle differentiation. The protein encoded by this gene binds GTP and is a member of the small GTPase superfamily. It is involved in endosome dynamics and reorganization of the actin cytoskeleton, and it may coordinate membrane transport with the function of the cytoskeleton. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

RHOD: Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton. Participates in reorganization of actin cytoskeleton. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein; G protein, monomeric; G protein, monomeric, Rho; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: cytosol; endosome membrane; plasma membrane

Biological Process: actin filament bundle formation; focal adhesion formation; lamellipodium biogenesis; positive regulation of cell adhesion; positive regulation of cell migration; regulation of small GTPase mediated signal transduction; Rho protein signal transduction