RGS1 recombinant protein

RGS1 recombinant protein

Cat. Num. AAA515377

• Gene Names: RGS1; 1R20; BL34; IER1; IR20
• Applications: Western Blot
• Synonyms: RGS1; RGS1 recombinant protein; IR20; IER1; 1R20; BL34

• Host: E Coli
• Form/Format: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Sequence Length: 1379

• Applicable Applications for RGS1 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: His tag
• Expression System: E.coli
• Source Note: Recombinant full-length human RGS1 Protein was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for RGS1 recombinant protein

Recombinant full-length human RGS1 Protein was expressed in E. coli cells using an N-terminal His tag.

Scientific Background: RGS1 is a member of the regulator of G-protein signalling family and attenuates the signalling activity of G-proteins. RGS1 binds to activated GTP-bound G alpha subunit and acts as a GTPase activating protein (GAP) thereby increasing the rate of conversion of GTP to GDP and terminating the signal (1). RGS1 is extensively up-regulated in renal cell carcinoma (RCC) tissues and melanoma. In melanoma, RGS1 expression is significantly correlated with increased tumor thickness, mitotic rate and presence of vascular involvement. Furthermore, there is significant association between increasing RGS1 expression and reduced relapse-free survival as well as disease-specific survival (DSS) survival (2).

Product Categories/Family for RGS1 recombinant protein

G-Proteins; Signaling Proteins - G-Proteins

References

1. Sierra D A, et al: Evolution of the regulators of G-protein signaling multigene family in mouse and human. Genomics 79: 177-185, 2002.
2. Rangel J, et al: Novel role for RGS1 in melanoma progression. Am J Surg Pathol. 2008 Aug;32(8):1207-12

NCBI and Uniprot Product Information

• NCBI GI #: 15930145
• NCBI GeneID: 5996
• UniProt Accession #: Q08116; Q07918; Q9H1W2; B2RDM9; B4DZY0
• Molecular Weight: ~26 kDa
• NCBI Official Full Name: Homo sapiens regulator of G-protein signaling 1, mRNA
• NCBI Official Synonym Full Names: regulator of G-protein signaling 1
• NCBI Official Symbol: RGS1
• NCBI Official Synonym Symbols: 1R20; BL34; IER1; IR20
• NCBI Protein Information: regulator of G-protein signaling 1; early response protein 1R20; B-cell activation protein BL34; regulator of G-protein signalling 1; immediate-early response 1, B-cell specific
• UniProt Protein Name: Regulator of G-protein signaling 1
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS1
• UniProt Synonym Gene Names: 1R20; BL34; IER1; RGS1
• UniProt Entry Name: RGS1_HUMAN

NCBI Description

This gene encodes a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. This protein may be involved in the regulation of B-cell activation and proliferation.

Tissue specificity: B-cell specific. Expression is relatively low in B-cells and chronic lymphocytic leukemia B-cells; however, in other types of malignant B-cell such as non-Hodgkin lymphoma and hairy cell leukemia, expression is constitutively high.

Induction: In response to several B-cell activation signals.

Post-translational modification: Could be phosphorylated. Might be functionally regulated by protein kinase(s).

Sequence similarities: Contains 1 RGS domain.

Sequence caution: The sequence AAB26289.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH15510.1 differs from that shown. Reason: Erroneous initiation. The sequence BAG37976.1 differs from that shown. Reason: Erroneous initiation. The sequence BAG64242.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA51826.1 differs from that shown. Reason: Erroneous initiation.