Goat anti-Human RGS1 Polyclonal Antibody | anti-RGS1 antibody

RGS1, CT (B-cell Activation Protein BL34, Early Response Protein 1R20, Regulator of G-protein Signaling 1, 1R20, BL34, IER1)

Cat. Num. AAA6011287

• Gene Names: RGS1; 1R20; BL34; IER1; IR20
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: RGS1; Polyclonal Antibody; CT (B-cell Activation Protein BL34; Early Response Protein 1R20; Regulator of G-protein Signaling 1; 1R20; BL34; IER1); Anti -RGS1; anti-RGS1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human RGS1. Species sequence homology: Mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-RGS1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.5-2ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
• Immunogen: Synthetic peptide corresponding to C-NLLNDLQANSLK of human RGS1 at C-terminal.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Small Intestine (formalin-fixed, paraffin-embedded) stained with RGS1 antibody followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Human Thymus (formalin-fixed, paraffin-embedded) stained with RGS1 antibody followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Western Blot (WB)

(Antibody staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Related Product Information for anti-RGS1 antibody

RGS1 is a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120aa motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal.

Product Categories/Family for anti-RGS1 antibody

Antibodies; Abs to G Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 115387108
• NCBI GeneID: 5996
• NCBI Accession #: NP_002913.3
• NCBI GenBank Nucleotide #: NM_002922.3
• UniProt Accession #: Q08116; Q07918; Q9H1W2; B2RDM9; B4DZY0
• Molecular Weight: 23,858 Da
• NCBI Official Full Name: regulator of G-protein signaling 1
• NCBI Official Synonym Full Names: regulator of G-protein signaling 1
• NCBI Official Symbol: RGS1
• NCBI Official Synonym Symbols: 1R20; BL34; IER1; IR20
• NCBI Protein Information: regulator of G-protein signaling 1; early response protein 1R20; B-cell activation protein BL34; regulator of G-protein signalling 1; immediate-early response 1, B-cell specific
• UniProt Protein Name: Regulator of G-protein signaling 1
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS1
• UniProt Synonym Gene Names: 1R20; BL34; IER1; RGS1
• UniProt Entry Name: RGS1_HUMAN

NCBI Description

This gene encodes a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. [provided by RefSeq, Jul 2008]

Uniprot Description

RGS1: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. This protein may be involved in the regulation of B-cell activation and proliferation. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: cytoplasm; plasma membrane

Molecular Function: calmodulin binding; GTPase activator activity

Biological Process: immune response; G-protein signaling, adenylate cyclase inhibiting pathway; signal transduction; positive regulation of GTPase activity