Mouse Galectin-7 ELISA Kit | LGALS7 elisa kit

Mouse Galectin-7 PicoKine ELISA Kit

Cat. Num. AAA177347

• Gene Names: Lgals7; Pig1; Galectin-7
• Reactivity: Mouse
• Synonyms: Galectin-7; Mouse Galectin-7 PicoKine ELISA Kit; lectin; galactoside-binding; soluble; 7; GAL7; HKL14; Human keratinocyte lectin 14; Lectin galactoside binding soluble 7; LGALS7; LGALS7B; MGC151215; MGC151217; OTTMUSP00000025970; OTTMUSP00000038956; p53 induced gene 1 protein; PI7; PIG1; TP53I1; LGALS7 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse Galectin-7
• Sequence Length: 136

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 93.8pg/ml-6000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse Galectin-7 PicoKine ELISA Kit standard curve )

Related Product Information for LGALS7 elisa kit

Principle of the assay: mouse Galectin-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Galectin-7 has been precoated onto 96-well plates. Standards(E.coli, S2-F136) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Galectin-7 amount of sample captured in plate.
Background: Galectin-7, also known as GAL7, is a protein that in humans is encoded by the LGALS7 gene. This gene is mapped to 19q13.2. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It has been found that expression of Galectin-7 was markedly upregulated in corneal epithelium after injury and that exogenous galectin-7 stimulated reepithelialization of corneal wounds.

NCBI and Uniprot Product Information

• NCBI GI #: 3913978
• NCBI GeneID: 16858
• UniProt Accession #: O54974
• Molecular Weight: 15,173 Da
• NCBI Official Full Name: Galectin-7
• NCBI Official Synonym Full Names: lectin, galactose binding, soluble 7
• NCBI Official Symbol: Lgals7
• NCBI Official Synonym Symbols: Pig1; Galectin-7
• NCBI Protein Information: galectin-7; gal-7
• UniProt Protein Name: Galectin-7
• Protein Family: Galectin
• UniProt Gene Name: Lgals7
• UniProt Synonym Gene Names: Gal-7
• UniProt Entry Name: LEG7_MOUSE

Uniprot Description

LGALS7B: Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.

Protein type: Cell adhesion; Apoptosis

Cellular Component: cytoplasm; extracellular region; nucleus

Molecular Function: carbohydrate binding

Biological Process: apoptosis