Human NK3 Homeobox Protein 1 (NKX3-1) ELISA Kit | NKX3-1 elisa kit

Human NK3 Homeobox Protein 1 (NKX3-1) ELISA Kit

Cat. Num. AAA2706217

• Gene Names: NKX3-1; NKX3; BAPX2; NKX3A; NKX3.1
• Reactivity: Human
• Synonyms: NK3 Homeobox Protein 1 (NKX3-1); Human NK3 Homeobox Protein 1 (NKX3-1) ELISA Kit; BAPX2; NKX3; NKX3.1; NKX3A; NK3 transcription factor related; locus 1; Homeobox protein NK-3 homolog A; NKX3-1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NK3 Homeobox Protein 1 (NKX3-1).; No significant cross-reactivity or interference between NK3 Homeobox Protein 1 (NKX3-1) and analogues was observed.
• Sequence Length: 190

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NK3 Homeobox Protein 1 (NKX3-1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NK3 Homeobox Protein 1 (NKX3-1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NKX3-1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NK3 Homeobox Protein 1 (NKX3-1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NK3 Homeobox Protein 1 (NKX3-1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NK3 Homeobox Protein 1 (NKX3-1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NK3 Homeobox Protein 1 (NKX3-1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 50960412
• NCBI GeneID: 4824
• Molecular Weight: 21,626 Da
• NCBI Official Full Name: NK3 homeobox 1
• NCBI Official Synonym Full Names: NK3 homeobox 1
• NCBI Official Symbol: NKX3-1
• NCBI Official Synonym Symbols: NKX3; BAPX2; NKX3A; NKX3.1
• NCBI Protein Information: homeobox protein Nkx-3.1
• UniProt Protein Name: Homeobox protein Nkx-3.1
• Protein Family: Homeobox protein
• UniProt Gene Name: NKX3-1
• UniProt Synonym Gene Names: NKX3.1; NKX3A

NCBI Description

This gene encodes a homeobox-containing transcription factor. This transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. Aberrant expression of this gene is associated with prostate tumor progression. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.