Rabbit anti-Human Connexin 40 Polyclonal Antibody | anti-Cx40 antibody

Connexin 40, NT (Gap Junction alpha-5 Protein, Connexin-40, Cx40, GJA5) (FITC)

Cat. Num. AAA6470137

• Gene Names: GJA5; CX40; ATFB11
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Connexin 40; Polyclonal Antibody; NT (Gap Junction alpha-5 Protein; Connexin-40; Cx40; GJA5) (FITC); anti-Cx40 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GJA5.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Cx40 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human GJA5 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-GJA5 N-term Pab in Placenta lysate. GJA5 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJA5 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-Cx40 antibody

Antibodies; Connexin; Junction Proteins

References

1. Dupays, L., et al., Gene 305(1):79-90 (2003). 2. Simon, A.M., et al., Dev. Biol. 251(2):206-220 (2002). 3. Cronier, L., et al., Mol. Hum. Reprod. 8(11):1005-1013 (2002). 4. Oviedo-Orta, E., et al., Immunology 99(4):578-590 (2000). 5. Gelb, B.D., et al., Genomics 39(3):409-411 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 8928556
• NCBI GeneID: 2702
• UniProt Accession #: P36382; Q5T3B6; Q5U0N6
• Molecular Weight: 40,380 Da
• NCBI Official Full Name: Gap junction alpha-5 protein
• NCBI Official Synonym Full Names: gap junction protein, alpha 5, 40kDa
• NCBI Official Symbol: GJA5
• NCBI Official Synonym Symbols: CX40; ATFB11
• NCBI Protein Information: gap junction alpha-5 protein; connexin 40; gap junction protein alpha 5 40kDa
• UniProt Protein Name: Gap junction alpha-5 protein
• UniProt Gene Name: GJA5
• UniProt Synonym Gene Names: Cx40
• UniProt Entry Name: CXA5_HUMAN

NCBI Description

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

GJA5: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJA5 are a cause of familial atrial standstill (FAS). Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm. Defects in GJA5 are the cause of familial atrial fibrillation type 11 (ATFB11). ATFB11 is a familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the connexin family. Alpha-type (group II) subfamily.

Protein type: Channel, misc.; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: connexon complex; cell projection; integral to plasma membrane; gap junction

Molecular Function: gap junction hemi-channel activity

Biological Process: gap junction assembly; negative regulation of glomerular filtration; positive regulation of vasodilation; protein oligomerization; positive regulation of vasoconstriction; artery morphogenesis; embryonic heart tube development; negative regulation of blood pressure; angiogenesis; skeletal development; transmembrane transport; potassium ion transport; embryonic limb morphogenesis

Disease: Atrial Standstill 1; Atrial Fibrillation, Familial, 11