Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Formylmethionine (fMET) Standard Curve.)
Human Formylmethionine (fMET) ELISA Kit | fMET elisa kit
Human Formylmethionine (fMET) ELISA Kit
Reactivity
Human
Synonyms
Formylmethionine (fMET); Human Formylmethionine (fMET) ELISA Kit; fMET elisa kit
Reactivity
Human
Specificity
Human Formylmethionine (fMET) ELISA Kit has high sensitivity and excellent specificity for detection of Human fMET. No significant cross-reactivity or interference between Human fMET and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Formylmethionine (fMET) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Formylmethionine (fMET) Standard Curve.)
Related Product Information for fMET elisa kit
Description: This Human Formylmethionine (fMET) ELISA Kit employs a two-site sandwich ELISA to quantitate fMET in samples. An antibody specific for fMET has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any fMET present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for fMET is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of fMET bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: fMet-Leu-Phe receptor is a protein encoded by the FPR1 gene.Ozcelik et al. mapped the FPR1 gene to human chromosome 19 by Southern analysis of rodent/human somatic cell hybrids and use of a cDNA probe. An FPR-like gene was also identified on chromosome 19. An FPR-like gene that mapped to chromosome 2 was found in fact to represent an interleukin 8 receptor. Bao et al. mapped the gene encoding the receptor for the chemotactic ligand FMLP to chromosome 19, using a panel of somatic cell hybrids. They identified 2 structural homologs of FPR that similarly mapped to chromosome 19. The structural homologs did not recognize the ligand FMLP, but were thought to represent chemotactic receptors. Bao et al. referred to them as orphan receptors and symbolized the genes FPRH1 and FPRH2.
Background: fMet-Leu-Phe receptor is a protein encoded by the FPR1 gene.Ozcelik et al. mapped the FPR1 gene to human chromosome 19 by Southern analysis of rodent/human somatic cell hybrids and use of a cDNA probe. An FPR-like gene was also identified on chromosome 19. An FPR-like gene that mapped to chromosome 2 was found in fact to represent an interleukin 8 receptor. Bao et al. mapped the gene encoding the receptor for the chemotactic ligand FMLP to chromosome 19, using a panel of somatic cell hybrids. They identified 2 structural homologs of FPR that similarly mapped to chromosome 19. The structural homologs did not recognize the ligand FMLP, but were thought to represent chemotactic receptors. Bao et al. referred to them as orphan receptors and symbolized the genes FPRH1 and FPRH2.
Similar Products
Product Notes
The Human fMET (Catalog #AAA9716276) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716276 ELISA Kit recognizes Human fMET. It is sometimes possible for the material contained within the vial of "Formylmethionine (fMET), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
