Rabbit anti-Human USP33 Polyclonal Antibody | anti-USP33 antibody

USP33, CT (Ubiquitin Carboxyl-terminal Hydrolase 33, Ubiquitin Thiolesterase 33, Ubiquitin-specific Processing Protease 33, Deubiquitinating Enzyme 33, VHL-interacting Deubiquitinating Enzyme 1, hVDU1, KIAA1097, VDU1) (Azide free) (HRP)

Cat. Num. AAA6505391

• Gene Names: USP33; VDU1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: USP33; Polyclonal Antibody; CT (Ubiquitin Carboxyl-terminal Hydrolase 33; Ubiquitin Thiolesterase 33; Ubiquitin-specific Processing Protease 33; Deubiquitinating Enzyme 33; VHL-interacting Deubiquitinating Enzyme 1; hVDU1; KIAA1097; VDU1) (Azide free) (HRP); anti-USP33 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VDU1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 942

• Applicable Applications for anti-USP33 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human VDU1-II (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-VDU1 Pab in T47D cell line lysate (35ug/lane). VDU1(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-USP33 antibody

Antibodies; Ubiquitin

References

1. Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). 2. Li, Z., et al., J. Biol. Chem. 277(7):4656-4662 (2002). 3. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 4. Kikuno, R., et al., DNA Res. 6(3):197-205 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 116242838
• NCBI GeneID: 23032
• UniProt Accession #: Q8TEY6; Q8TEY7
• Molecular Weight: 106.752
• NCBI Official Full Name: Ubiquitin carboxyl-terminal hydrolase 33
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 33
• NCBI Official Symbol: USP33
• NCBI Official Synonym Symbols: VDU1
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 33
• Protein Family: Ubiquitin carboxyl-terminal hydrolase

NCBI Description

This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012]