Kremen2, Rat Active Protein | Kremen2 active protein

Kremen2, Recombinant, Rat (Kringle-containing Protein Marking the Eye and the Nose, Kringle Domain-containing Transmembrane Protein 2)

Cat. Num. AAA636198

• Purity: Purified; 85%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Synonyms: Kremen2; Rat; Recombinant; Rat (Kringle-containing Protein Marking the Eye and the Nose; Kringle Domain-containing Transmembrane Protein 2); Kremen2 active protein

• Host: Recombinant Rat
• Purity/Purification: Purified; 85%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.

• Activity: Measured by its ability to bind biotinylated rhDkk-1.
• Endotoxin Level: 1EU/1ug.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Kremen2 active protein

Kremen (Kringle-containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains, and an intracellular region with no conserved motifs (1). Two related molecules, Kremen-1 and -2, have been identified. Kremens bind with high affinity to a subset of the secreted Dickkopf proteins (Dkk-1, -2, and -4) to modulate the canonical Wnt signaling pathway (2). This pathway is transduced by a ternary receptor complex composed of Wnt, the seven-transmembrane domain receptor Frizzled, and the LDL-receptor-related protein 5/6 (LRP5/6) coreceptor (3, 4). Dkk-1 and -4 bind directly to the LRP5/6 coreceptor to antagonize the canonical Wnt/b-catenin signaling pathway. The planar cell polarity (PCP) signaling pathway is not affected as it does not involve LRP5/6 (2). In contrast, Dkk-3 has no effect on Wnt signaling. Dkk-2 can function either as an LRP agonist or antagonist, depending on whether or not the cell expresses Kremen (5). Kremen cooperates with Dkk to antagonize Wnt signaling via formation of a Kremen-Dkk-LRP ternary complex which is internalized and cleared from the cell surface (4). Binding requires all three extracellular domains of Kremen, and the second cysteine-rich domain of Dkk (4). The predicted rat Kremen-2 cDNA encodes a 490 aa glycosylated protein with a 51 aa signal peptide, a 340 aa extracellular domain, a 24 aa transmembrane domain, and a 75 aa cytoplasmic domain. Within the ECD, rat Kremen-2 shares 91% and 98% aa sequence identity with human and mouse Kremen-2, respectively, and 46% with rat Kremen-1.

A DNA sequence encoding the extracellular domain of rat Kremen-2 (Gly 53-Arg 395; Accession # XP_220206) was fused with a six-histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.
Calculated molecular mass of ~ 37.5kD. As a result of glycosylation, recombinant rat Kremen-2 migrates as an ~ 45-55kD protein in SDS-PAGE under reducing conditions.

Product Categories/Family for Kremen2 active protein

Molecular Biology; MB-Dickkopf Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 157786772
• NCBI GeneID: 287102
• NCBI Accession #: NP_001099237.1
• NCBI GenBank Nucleotide #: NM_001105767.1
• NCBI Official Full Name: kremen protein 2
• NCBI Official Synonym Full Names: kringle containing transmembrane protein 2
• NCBI Official Symbol: Kremen2
• NCBI Protein Information: kremen protein 2
• Protein Family: Kremen protein