Interleukin 6 Active Protein | Il6 active protein
Interleukin 6, Recombinant, Human (IL-6)
Gene Names
Il6; ILg6; Ifnb2
Purity
Highly Purified98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: 0.1ng/ug (IEU/ug)
Synonyms
Interleukin 6; Recombinant; Human (IL-6); Il6 active protein
Host
Recombinant, Human (E Coli)
Purity/Purification
Highly Purified
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: 0.1ng/ug (IEU/ug)
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: 0.1ng/ug (IEU/ug)
Form/Format
Supplied as a lyophilized powder in 1.6% HSA and 2% mannitol.
Sequence
Met-Pro-Val-Pro-Pro (first 5 N-terminal aa)
Biological Activity
rHuIL-6 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine 7TD1 cells is less then 0.1ng/ml, corresponding to a Specific Activity of ~1 x 10e6 IU/mg.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
1. UV spectroscopy at 280nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding ddH2O, 0.1% HSA or BSA and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Il6 active protein
Recombinant Human IL-6 is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21kD.
Product Categories/Family for Il6 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21kD
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
ILg6; Ifnb2
NCBI Protein Information
interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)
UniProt Protein Name
Interleukin-6
Protein Family
UniProt Gene Name
Il6
UniProt Synonym Gene Names
Il-6
UniProt Entry Name
IL6_RAT
NCBI Description
a cytokine involved in development and possibly in neurodegenerative processes [RGD]
Uniprot Description
Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance
Subcellular location: Secreted.
Sequence similarities: Belongs to the IL-6 superfamily.
Research Articles on Il6
Similar Products
Product Notes
The Il6 il6 (Catalog #AAA650909) is an Active Protein produced from Recombinant, Human (E Coli) and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met-Pro-Va l-Pro-Pro (first 5 N-terminal aa). It is sometimes possible for the material contained within the vial of "Interleukin 6, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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