CSF1R active protein

CSF1R Protein, Rat, Recombinant (hFc Tag)

Cat. Num. AAA8123477

• Gene Names: Csf1r; c-fms; mrfms; CSF-1R; CSF-1-R; M-CSF-R
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CSF1R; CSF1R Protein; Rat; Recombinant (hFc Tag); Rat CSF1R/MCSF Receptor/CD115 Protein (Fc Tag); colony stimulating factor 1 receptor; CSF1R active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu510

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 20
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the rat CSF1R (D4ACA7)(Met1-Glu510) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Immobilized rat CSF1R-Fc at 10ug/ml (100uL/well) can bind biotinylated human CSF1-His. The EC50 of biotinylated human CSF1-His is 1.7-3.9ng/mL.; 2. Measured by its ability to inhibit mouse CSF induced proliferation of M?NFS?60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 50-250ng/mL in the presence of 2.5ng/mL. Recombinant Mouse M-CSF.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to inhibit mouse CSF induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 50-250 ng/mL in the presence of 2.5 ng/mL Recombinant Mouse M-CSF.)

Testing Data

(Measured by its ability to inhibit mouse CSF induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 50-250 ng/mL in the presence of 2.5 ng/mL Recombinant Mouse M-CSF.)

Related Product Information for CSF1R active protein

Background: M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages. This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. Binding of CSF1 activates the receptor kinase, leading to "autophosphorylation" of receptor subunits and the concomitant phosphorylation of a series of cellular proteins on tyrosine residues. CSF1R is a tyrosine kinase receptor that is absolutely required for macrophage differentiation and thus occupies a central role in hematopoiesis. CSF1 and its receptor (CSF1R, product of c-fms proto-oncogene) were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role for CSF1/CSF1R in normal mammary gland development is very intriguing because this receptor/ligand pair has also been found to be important in the biology of breast cancer in which abnormal expression of CSF1 and its receptor correlates with tumor cell invasiveness and adverse clinical prognosis. Tumor cell expression of CSF1R is under the control of several steroid hormones (glucocorticoids and progestins) and the binding of several bHLH transcription factors, while tumor cell expression of CSF-1 appears to be regulated by other hormones, some of which are involved in normal lactogenic differentiation. However, studies have demonstrated that CSF1 and CSF1R have additional roles in mammary gland development during pregnancy and lactation. The role of CSF1 and CSF1R in normal and neoplastic mammary development that may elucidate potential relationships of growth factor-induced biological changes in the breast during pregnancy and tumor progression.

Product Categories/Family for CSF1R active protein

Receptor Tyrosine Kinases (RTKs) Cancer Drug Targets; AKT Signaling Pathway

References

Sherr CJ. (1990) The colony-stimulating factor 1 receptor: pleiotropy of signal-response coupling. Lymphokine Res. 9(4): 543-8.Kacinski BM. (1997) CSF-1 and its receptor in breast carcinomas and neoplasms of the female reproductive tract. Mol Reprod Dev. 46(1): 71-4.Sapi E, et al. (1999) The role of CSF-1 in normal and neoplastic breast physiology. Proc Soc Exp Biol Med. 220(1): 1-8.Sapi E. (2004) The role of CSF-1 in normal physiology of mammary gland and breast cancer: an update. Exp Biol Med (Maywood). 229(1): 1-11.Bonifer C, et al. (2008) The transcriptional regulation of the Colony-Stimulating Factor 1 Receptor (csf1r) gene during hematopoiesis. Front Biosci. 13: 549-60.

NCBI and Uniprot Product Information

• NCBI GI #: 71361659
• NCBI GeneID: 307403
• NCBI Accession #: NP_001025072.1
• NCBI GenBank Nucleotide #: NM_001029901.1
• UniProt Accession #: D4ACA7; Q00495
• Molecular Weight: 114,164 Da
• NCBI Official Full Name: macrophage colony-stimulating factor 1 receptor
• NCBI Official Synonym Full Names: colony stimulating factor 1 receptor
• NCBI Official Symbol: Csf1r
• NCBI Official Synonym Symbols: c-fms; mrfms; CSF-1R; CSF-1-R; M-CSF-R
• NCBI Protein Information: macrophage colony-stimulating factor 1 receptor
• UniProt Protein Name: Tyrosine-protein kinase receptor
• Protein Family: Macrophage colony-stimulating factor 1 receptor
• UniProt Gene Name: Csf1r

NCBI Description

may mediate normal and neoplastic growth of muscular cells [RGD, Feb 2006]