High security: Add unique non-toxic optimized ingredients/No need for decolorization, and no need for immobilization of methanol and ethanol.
High sensitivity: Add signal-enhancing dye components/Can detect proteins as low as 10 ng.
Easy to operate: Ready to use/No need to prepare, can be used directly.
SDS-PAGE gel electrophoresis, Non-denaturing PAGE gel electrophoresis.
When using Protein Gel Fast Staining Solution, you need to prepare deionized water and Staining container before the experiments.
The washing and Staining time mentioned in the experimental steps are applicable to the gel with a thickness of 0.75 mm to 1.5 mm. For the thicker gel, the dyeing time can be appropriately extended according to the color rendering effect of the target protein band.
This Staining solution is acidic and slightly corrosive. Wear lab coat, gloves and other protective equipment when using.
Residual impurities can increase the staining background and reduce target protein resolution, so it is necessary to wash thoroughly with deionized water before staining to remove impurities.
Shipping||Gel pack with blue ice.
Testing Data
(Fig.1 SuperKine Protein Gel Fast Staining Solution (Coomassie Blue) was used to stained BSA and compared with staining solutions from A manufcturer and B manufcturer. The sample loading are Marker, 10 ng, 20 ng, 50 ng, 100 ng, 200 ng, 500 ng, 1000 ng, 2000 ng, 5000 ng, Marker respectively. The SuperKine Protein Gel Fast Staining Solution (Coomassie Blue) from showed much clearer and cleaner results, no matter they were stained for 15 min or 1 h.)
Background: Coomassie blue is a commonly used dye for the visualization of proteins (separated by protein gel electrophoresis). Under acidic conditions, Coomassie blue binds to the alkaline and hydrophobic amino acid residues of the protein, and the color is dark blue. The ready to use SuperKine Protein Gel Fast Staining Solution (Coomassie Blue) developed by has the advantages of short dyeing time, clear color rendering, good repeatability and high safety, and a clear and clean background can be obtained without decolorization. Based on the classical Coomassie blue staining principle, the target proteins recovered after staining can be used for subsequent mass spectrometry experiments or sequencing analysis.
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Product Notes
The Protein Gel Fast Staining Solution (Catalog #AAA9719198) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Protein Gel Fast Staining Solution, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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