Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using LIG1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Rabbit anti-Human, Mouse LIG1 Polyclonal Antibody | anti-LIG1 antibody
LIG1 Polyclonal Antibody
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using LIG1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using LIG1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cells using LIG1 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cells using LIG1 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells using LIG1 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells using LIG1 antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Uniprot Description
LIG1: is an enzyme that functions in DNA replication and the base excision repair process. Mutations leading to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents.
Protein type: Ligase; EC 6.5.1.1; DNA repair, damage
Chromosomal Location of Human Ortholog: 19q13.2-q13.3
Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; mitochondrion; chromosome; nucleus
Molecular Function: DNA binding; DNA ligase activity; metal ion binding; DNA ligase (ATP) activity; ATP binding
Biological Process: anatomical structure morphogenesis; mismatch repair; V(D)J recombination; DNA strand elongation during DNA replication; DNA repair; lagging strand elongation; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; telomere maintenance via semi-conservative replication; response to hydrogen peroxide; base-excision repair; nucleotide-excision repair; cell division; transcription-coupled nucleotide-excision repair; double-strand break repair; telomere maintenance via recombination; DNA ligation during DNA repair; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; telomere maintenance; DNA metabolic process
Research Articles on LIG1
Similar Products
Product Notes
The LIG1 lig1 (Catalog #AAA127265) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LIG1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LIG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the LIG1 lig1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
