Rabbit anti-Mouse, Rat RIP2 Polyclonal Antibody | anti-RIP2 antibody

RABBIT ANTI RIP2 (N-TERMINAL)

Cat. Num. AAA223329

• Gene Names: RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: RIP2; Polyclonal Antibody; RABBIT ANTI RIP2 (N-TERMINAL); anti-RIP2 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5 mg/ml (varies by lot)
• Sequence Length: 540

• Applicable Applications for anti-RIP2 antibody: Immunohistology Frozen, Western Blot (WB)
• Application Notes: Western Blot: Rabbit anti Human RIP2 detects a band of approximately 60kDa in A431 cell lysates.; Immunohistology - Frozen: Maximum Dilution: 10ug/ml; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Histology Positive Control Tissue: Placenta; Buffer Solution
• Antiserum Preparation: Antiserum to human RIP2 (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysates from A431 (A) and K562 (K) cells probed with Rabbit anti RIP-2 (MBS223329))

Testing Data #2

(Immunocytochemical staining of A431 cells with Rabbit anti RIP-2 (MBS223329))

Related Product Information for anti-RIP2 antibody

Rabbit anti Human RIP2 antibody recognizes Human Receptor-interacting serine/threonine-protein kinase 2, also known as RIP2, RIPK2, RIP-like-interacting CLARP kinase or CARD-containing interleukin-1 beta-converting enzyme-associated kinase. RIP2 is a 403 amino acid ~60 kDa cytoplasmic protein belonging to the protein kinase superfamily. RIP2 is a kinase that regulates TNF and Fas induced-apoptosis and is involved in the generation of the pro-inflammatory cytokine IL-1beta. Over expression of RIP2 has been shown to induce apoptosis, and the activation of NF-Kappa-B and JNK. RIP2 is widely expressed in a range of human tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 4506537
• NCBI GeneID: 8767
• NCBI Accession #: NP_003812.1
• NCBI GenBank Nucleotide #: NM_003821.5
• UniProt Accession #: O43353; Q6UWF0; B7Z748
• Molecular Weight: 45,582 Da
• NCBI Official Full Name: receptor-interacting serine/threonine-protein kinase 2
• NCBI Official Synonym Full Names: receptor-interacting serine-threonine kinase 2
• NCBI Official Symbol: RIPK2
• NCBI Official Synonym Symbols: CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 2; CARD-carrying kinase; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; RIP-2; growth-inhibiting gene 30; receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase; receptor-interacting protein 2; tyrosine-protein kinase RIPK2
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 2
• Protein Family: Putative zinc metalloprotease
• UniProt Gene Name: RIPK2
• UniProt Synonym Gene Names: CARDIAK; RICK; RIP2; CARD-containing IL-1 beta ICE-kinase; RIP-2
• UniProt Entry Name: RIPK2_HUMAN

NCBI Description

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq, Jul 2008]

Uniprot Description

RIPK2: a tyrosine kinase-like kinase of the RIPK family. Activates pro-caspase-1 and pro-caspase-8. Potentiates casp-8-mediated apoptosis. May activate NF-kappaB.

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TKL; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); TKL group; RIPK family

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: cytoskeleton; protein complex; cytoplasm; cytosol; vesicle

Molecular Function: protein serine/threonine kinase activity; LIM domain binding; signal transducer activity; protein binding; protein homodimerization activity; CARD domain binding; non-membrane spanning protein tyrosine kinase activity; ATP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; activation of MAPK activity; positive regulation of interleukin-12 production; apoptosis; positive regulation of apoptosis; stress-activated MAPK cascade; positive regulation of JNK cascade; toll-like receptor 3 signaling pathway; signal transduction; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; T cell proliferation; response to exogenous dsRNA; positive regulation of immature T cell proliferation; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon-beta production; JNK cascade; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; adaptive immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of peptidyl-serine phosphorylation; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; positive regulation of interferon-gamma production; positive regulation of protein ubiquitination; toll-like receptor signaling pathway; positive regulation of interleukin-2 production; innate immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; positive regulation of T-helper 1 cell differentiation; positive regulation of cytokine and chemokine mediated signaling pathway; negative regulation of apoptosis