Human RNA Binding Motif Protein 8 ELISA Kit | RBM8 elisa kit

Human RNA Binding Motif Protein 8 (RBM8) ELISA Kit

Cat. Num. AAA2031876

• Gene Names: RBM8A; TAR; Y14; RBM8; ZNRP; RBM8B; ZRNP1; BOV-1A; BOV-1B; BOV-1C; MDS014; DEL1q21.1; C1DELq21.1
• Reactivity: Human
• Synonyms: RNA Binding Motif Protein 8; Human RNA Binding Motif Protein 8 (RBM8) ELISA Kit; RBM8A; BOV-1A; BOV-1B; BOV-1C; RBM8B; Y14; ZNRP; ZRNP1; Binder of OVCA1-1; RNA-binding protein Y14; Ribonucleoprotein RBM8A; RBM8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RNA Binding Motif Protein 8 (RBM8).; No significant cross-reactivity or interference between RNA Binding Motif Protein 8 (RBM8) and analogues was observed.
• Sequence Length: 173

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.063ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RNA Binding Motif Protein 8 (RBM8) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RNA Binding Motif Protein 8 (RBM8) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Related Product Information for RBM8 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RNA Binding Motif Protein 8 (RBM8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RNA Binding Motif Protein 8 (RBM8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNA Binding Motif Protein 8 (RBM8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RNA Binding Motif Protein 8 (RBM8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RBM8 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 7158824
• NCBI GeneID: 9939
• Molecular Weight: 19,760 Da
• NCBI Official Full Name: RNA binding motif protein 8
• NCBI Official Synonym Full Names: RNA binding motif protein 8A
• NCBI Official Symbol: RBM8A
• NCBI Official Synonym Symbols: TAR; Y14; RBM8; ZNRP; RBM8B; ZRNP1; BOV-1A; BOV-1B; BOV-1C; MDS014; DEL1q21.1; C1DELq21.1
• NCBI Protein Information: RNA-binding protein 8A
• UniProt Protein Name: RNA-binding protein 8A
• UniProt Gene Name: RBM8A
• UniProt Synonym Gene Names: RBM8; BOV-1
• UniProt Entry Name: RBM8A_HUMAN

NCBI Description

This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. There are two alternate translation start codons with this gene, which result in two forms of the protein. An allele mutation and a low-frequency noncoding single-nucleotide polymorphism (SNP) in this gene cause thrombocytopenia-absent radius (TAR) syndrome. [provided by RefSeq, Jul 2013]

Uniprot Description

RBM8A: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Remains associated with mRNAs in the cytoplasm until the mRNAs engage the translation machinery. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Complex with MAGOH is a component of the nonsense mediated decay (NMD) pathway. Belongs to the RBM8A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: cell soma; cytoplasm; cytosol; dendrite; nuclear speck; nucleoplasm; nucleus

Molecular Function: mRNA binding; nucleotide binding; protein binding; RNA binding

Biological Process: gene expression; mRNA 3'-end processing; mRNA catabolic process, nonsense-mediated decay; mRNA export from nucleus; nuclear mRNA splicing, via spliceosome; regulation of alternative nuclear mRNA splicing, via spliceosome; regulation of translation; RNA splicing; termination of RNA polymerase II transcription; transcription from RNA polymerase II promoter

Disease: Thrombocytopenia-absent Radius Syndrome