Rat Deiodinase, Iodothyronine, Type II (DIO2) RTU ELISA Kit | DIO2 rtu elisa kit

Rat Deiodinase, Iodothyronine, Type II (DIO2) ELISA Kit

Cat. Num. AAA458858

• Gene Names: Dio2; 5DII; DIOII
• Reactivity: Rat
• Synonyms: Deiodinase; Iodothyronine; Type II (DIO2); Rat Deiodinase; Type II (DIO2) ELISA Kit; 5DII; D2; SelY; TXDI2; Thyroxine Deiodinase; Type II; Type-II 5'-deiodinase; DIO2 rtu elisa kit

• Reactivity: Rat
• Sequence Length: 266

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.781-50ng/mL
• Sensitivity: 0.24ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DIO2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DIO2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DIO2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DIO2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DIO2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 132566529
• NCBI GeneID: 65162
• NCBI Accession #: NP_113908.3
• NCBI GenBank Nucleotide #: NM_031720.4
• UniProt Accession #: P70551; O70179
• Molecular Weight: 29,871 Da
• NCBI Official Full Name: type II iodothyronine deiodinase
• NCBI Official Synonym Full Names: iodothyronine deiodinase 2
• NCBI Official Symbol: Dio2
• NCBI Official Synonym Symbols: 5DII; DIOII
• NCBI Protein Information: type II iodothyronine deiodinase
• UniProt Protein Name: Type II iodothyronine deiodinase
• Protein Family: Type II iodothyronine deiodinase
• UniProt Gene Name: Dio2
• UniProt Synonym Gene Names: Itdi2; Txdi2

NCBI Description

The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This gene is expressed in a few tissues in rat, including brain, pituitary and brown adipose tissue. It is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues. Studies in rat suggest that this gene may play an important role in the regulation of lipid metabolism and thermogenesis, and in osteoarthritis pathogenesis by enhancing the chondrocyte hypertrophy and inflammatory response. This protein is a selenoprotein containing the rare selenocysteine (Sec) amino acid at its active site, and may contain additional Sec residues. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, May 2016]

Uniprot Description

Catalyzes the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.