Typical Testing Data/Standard Curve
Canine Type II iodothyronine deiodinase ELISA Kit | DIO2 elisa kit
Canine Type II iodothyronine deiodinase ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay: DIO2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DIO2 antibody and an DIO2-HRP conjugate. The assay sample and buffer are incubated together with DIO2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DIO2 concentration since DIO2 from samples and DIO2-HRP conjugate compete for the anti-DIO2 antibody binding site. Since the number of sites is limited, as more sites are occupied by DIO2 from the sample, fewer sites are left to bind DIO2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DIO2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It is highly expressed in the thyroid, and may contribute significantly to the relative increase in thyroidal T3 production in patients with Graves disease and thyroid adenomas. This protein contains selenocysteine (Sec) residues encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
DIO2: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development. Belongs to the iodothyronine deiodinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.97.1.10; Oxidoreductase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 14q24.2-q24.3
Cellular Component: membrane; integral to membrane; plasma membrane
Molecular Function: thyroxine 5'-deiodinase activity; selenium binding; ubiquitin protein ligase binding
Biological Process: thyroid hormone generation; hormone biosynthetic process; selenocysteine incorporation; thyroid hormone metabolic process
Research Articles on DIO2
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Product Notes
The Canine DIO2 dio2 (Catalog #AAA747487) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA747487 ELISA Kit recognizes Canine DIO2. It is sometimes possible for the material contained within the vial of "Type II iodothyronine deiodinase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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