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Testing Data (Figure A. RPE-Cy5 tandem conjugated Mouse anti Human CD3 and A488 conjugated Mouse IgG1 isotype control . Figure B. RPE-Cy5 tandem conjugated Mouse anti Human CD3 and A488 conjugated Mouse anti Human CD38 . All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock .)

IgG1 negative control

Mouse IgG1 Negative Control: Amethyst Orange

Applications
Flow Cytometry, Functional Assay
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Synonyms
IgG1; Mouse IgG1 Negative Control: Amethyst Orange; Mouse IgG1 Negative Control antibody; IgG1 negative control
Ordering
For Research Use Only!
Host
Mouse
Isotype
IgG1
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Form/Format
Purified IgG conjugated to Amethyst Orange - Liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Applicable Applications for IgG1 negative control
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Buffer Solution
Phosphate Buffered Saline
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
This product contains a precipitate we recommend microcentrifugation before use.

Testing Data

(Figure A. RPE-Cy5 tandem conjugated Mouse anti Human CD3 and A488 conjugated Mouse IgG1 isotype control . Figure B. RPE-Cy5 tandem conjugated Mouse anti Human CD3 and A488 conjugated Mouse anti Human CD38 . All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock .)

Testing Data (Figure A. RPE-Cy5 tandem conjugated Mouse anti Human CD3 and A488 conjugated Mouse IgG1 isotype control . Figure B. RPE-Cy5 tandem conjugated Mouse anti Human CD3 and A488 conjugated Mouse anti Human CD38 . All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock .)

Testing Data

(Figure A. A488 conjugated Mouse anti Human CD38 and RPE-A647 tandem conjugated Mouse IgG1 isotype control . Figure B. A488 conjugated Mouse anti Human CD38 and RPE-Cy5 tandem conjugated Mouse anti Human CD3 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)

Testing Data (Figure A. A488 conjugated Mouse anti Human CD38 and RPE-A647 tandem conjugated Mouse IgG1 isotype control . Figure B. A488 conjugated Mouse anti Human CD38 and RPE-Cy5 tandem conjugated Mouse anti Human CD3 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)

Testing Data

(Figure A. FITC conjugated Mouse anti Human HLA DP/DQ/DR antibody and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human HLA DP/DQ/DR antibody and RPE conjugated Mouse anti Human CD11c . All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock )

Testing Data (Figure A. FITC conjugated Mouse anti Human HLA DP/DQ/DR antibody and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human HLA DP/DQ/DR antibody and RPE conjugated Mouse anti Human CD11c . All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock )

Testing Data

(Figure A. FITC conjugated Mouse anti Human CD3 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD3 and RPE conjugated Mouse anti Human CD18 . All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock .)

Testing Data (Figure A. FITC conjugated Mouse anti Human CD3 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD3 and RPE conjugated Mouse anti Human CD18 . All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock .)

Testing Data

(Figure A. RPE conjugated Mouse anti Human CD18 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD18 and FITC conjugated Mouse anti Human CD3 . All experiments performed on human Peripheral blood mononuclear cells in the presence of human SeroBlock .)

Testing Data (Figure A. RPE conjugated Mouse anti Human CD18 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD18 and FITC conjugated Mouse anti Human CD3 . All experiments performed on human Peripheral blood mononuclear cells in the presence of human SeroBlock .)

Testing Data

(Figure A. RPE conjugated Rat anti Human CD28 and A647 conjugated Mouse IgG1 istoype control . Figure B. RPE conjugated Rat anti Human CD28 and A647 conjugated Mouse anti Human CD4 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)

Testing Data (Figure A. RPE conjugated Rat anti Human CD28 and A647 conjugated Mouse IgG1 istoype control . Figure B. RPE conjugated Rat anti Human CD28 and A647 conjugated Mouse anti Human CD4 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)

Testing Data

(Figure A. APC conjugated Mouse anti Human CD3 and RPE conjugated Mouse IgG1 isotype control . Figure B. APC conjugated Mouse anti Human CD3 and RPE conjugated Mouse anti Human CD46 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)

Testing Data (Figure A. APC conjugated Mouse anti Human CD3 and RPE conjugated Mouse IgG1 isotype control . Figure B. APC conjugated Mouse anti Human CD3 and RPE conjugated Mouse anti Human CD46 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)

Testing Data

(Figure A. RPE conjugated Mouse anti Human CD46 and APC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD46 and APC conjugated Mouse anti Human CD3 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)

Testing Data (Figure A. RPE conjugated Mouse anti Human CD46 and APC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD46 and APC conjugated Mouse anti Human CD3 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)

Testing Data

(Figure A. RPE conjugated Rat anti Human CD49f and A488 conjugated Mouse IgG1 control . Figure B. RPE conjugated Rat anti Human CD49f and A488 conjugated Mouse anti Human CD3 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)

Testing Data (Figure A. RPE conjugated Rat anti Human CD49f and A488 conjugated Mouse IgG1 control . Figure B. RPE conjugated Rat anti Human CD49f and A488 conjugated Mouse anti Human CD3 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)

Testing Data

(Figure A. RPE conjugated Mouse anti Human CD59 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD59 and FITC conjugated Mouse anti Human CD3 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)

Testing Data (Figure A. RPE conjugated Mouse anti Human CD59 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD59 and FITC conjugated Mouse anti Human CD3 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)
Related Product Information for IgG1 negative control
Mouse IgG1 negative control is negative by flow cytometry on all human cells and cell lines tested. Further tests have also shown that this reagent is also suitable for use as a negative control with bovine (Maslanka et al, 2012), ovine, porcine (Kapetanovic et al, 2012), equine (Jacks et al, 2007), canine (Maiolini et al, 2012), lapine (Pakandl et al, 2008) and guinea-pig tissues.
Product Categories/Family for IgG1 negative control

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Product Notes

The IgG1 (Catalog #AAA225866) is a Negative Control produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IgG1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Researchers should empirically determine the suitability of the IgG1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG1, Negative Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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