Rat VGF Nerve Growth Factor Inducible (VGF) ELISA Kit | VGF elisa kit

Rat VGF Nerve Growth Factor Inducible, VGF ELISA Kit

Cat. Num. AAA911284

• Reactivity: Rat
• Synonyms: VGF Nerve Growth Factor Inducible (VGF); Rat VGF Nerve Growth Factor Inducible; VGF ELISA Kit; Rat VGF Nerve Growth Factor Inducible (VGF) ELISA Kit; neuro-endocrine specific protein VGF; neurosecretory protein VGF; VGF elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat VGF. No significant cross-reactivity or interference between Rat VGF and analogues was observed.
• Sequence Length: 617

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for VGF elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for VGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain VGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of VGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13591864
• NCBI GeneID: 29461
• NCBI Accession #: NP_112259.1
• NCBI GenBank Nucleotide #: NM_030997.1
• UniProt Accession #: P20156
• Molecular Weight: 68,179 Da
• NCBI Official Full Name: neurosecretory protein VGF
• NCBI Official Synonym Full Names: VGF nerve growth factor inducible
• NCBI Official Symbol: Vgf
• NCBI Protein Information: neurosecretory protein VGF; VGF8a protein
• UniProt Protein Name: Neurosecretory protein VGF
• Protein Family: Neurosecretory protein
• UniProt Gene Name: Vgf
• UniProt Synonym Gene Names: NERP-1; NERP-2
• UniProt Entry Name: VGF_RAT

NCBI Description

peptide growth factor that plays a key role in neuronal differentiation and survival; may regulate synaptic function in hippocampal neurons [RGD, Feb 2006]

Uniprot Description

VGF: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; transport vesicle; neuron projection; cell soma; secretory granule membrane; cell; extracellular region; cytoplasmic vesicle

Molecular Function: neuropeptide hormone activity; growth factor activity

Biological Process: neuron differentiation; response to dietary excess; generation of precursor metabolites and energy; response to cAMP; ovarian follicle development; sexual reproduction; insulin secretion; response to cold; glucose homeostasis; regulation of neuronal synaptic plasticity; penile erection; response to insulin stimulus