Rat VGF Nerve Growth Factor Inducible (VGF) ELISA Kit | VGF elisa kit

Rat VGF Nerve Growth Factor Inducible (VGF) ELISA Kit

Cat. Num. AAA2024640

• Gene Names: VGF; SCG7; SgVII
• Reactivity: Rat
• Synonyms: VGF Nerve Growth Factor Inducible (VGF); Rat VGF Nerve Growth Factor Inducible (VGF) ELISA Kit; NERP1; NERP2; Neuro-Endocrine Specific Protein VGF; Neuroendocrine Regulatory Peptide; VGF elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of VGF Nerve Growth Factor Inducible (VGF).; No significant cross-reactivity or interference between VGF Nerve Growth Factor Inducible (VGF) and analogues was observed.
• Sequence Length: 615

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 46.88-3000pg/mL
• Sensitivity: < 18.34pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VGF Nerve Growth Factor Inducible (VGF) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VGF Nerve Growth Factor Inducible (VGF) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VGF elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VGF Nerve Growth Factor Inducible (VGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VGF Nerve Growth Factor Inducible (VGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VGF Nerve Growth Factor Inducible (VGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VGF Nerve Growth Factor Inducible (VGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for VGF elisa kit

Metabolic pathway; Infection immunity; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 119570586
• NCBI GeneID: 7425
• UniProt Accession #: O15240; Q9UDW8
• Molecular Weight: 67,258 Da
• NCBI Official Full Name: VGF nerve growth factor inducible
• NCBI Official Synonym Full Names: VGF nerve growth factor inducible
• NCBI Official Symbol: VGF
• NCBI Official Synonym Symbols: SCG7; SgVII
• NCBI Protein Information: neurosecretory protein VGF
• UniProt Protein Name: Neurosecretory protein VGF
• Protein Family: Neurosecretory protein
• UniProt Gene Name: VGF
• UniProt Synonym Gene Names: NERP-1; NERP-2
• UniProt Entry Name: VGF_HUMAN

NCBI Description

This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

VGF: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular space; transport vesicle; cytoplasmic vesicle

Molecular Function: neuropeptide hormone activity; growth factor activity

Biological Process: response to dietary excess; generation of precursor metabolites and energy; response to cAMP; ovarian follicle development; insulin secretion; sexual reproduction; defense response to bacterium; response to cold; glucose homeostasis; response to insulin stimulus