Human VGF nerve growth factor inducible ELISA Kit | VGF elisa kit

Human VGF nerve growth factor inducible, VGF ELISA Kit

Cat. Num. AAA928603

• Reactivity: Human
• Synonyms: VGF nerve growth factor inducible; Human VGF nerve growth factor inducible; VGF ELISA Kit; Human VGF nerve growth factor inducible (VGF) ELISA kit; neuro-endocrine specific protein VGF; neurosecretory protein VGF; VGF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human VGF. No significant cross-reactivity or interference between human VGF and analogues was observed.
• Sequence Length: 615

• Samples: Serum, plasma, cell culture supernates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: < 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VGF elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 17136078
• NCBI GeneID: 7425
• NCBI Accession #: NP_003369.2
• NCBI GenBank Nucleotide #: NM_003378.3
• UniProt Accession #: O15240; Q9UDW8
• Molecular Weight: 67,258 Da
• NCBI Official Full Name: neurosecretory protein VGF
• NCBI Official Synonym Full Names: VGF nerve growth factor inducible
• NCBI Official Symbol: VGF
• NCBI Protein Information: neurosecretory protein VGF; neuro-endocrine specific protein VGF
• UniProt Protein Name: Neurosecretory protein VGF
• Protein Family: Neurosecretory protein
• UniProt Gene Name: VGF
• UniProt Synonym Gene Names: NERP-1; NERP-2
• UniProt Entry Name: VGF_HUMAN

NCBI Description

This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

VGF: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular space; transport vesicle; cytoplasmic vesicle

Molecular Function: neuropeptide hormone activity; growth factor activity

Biological Process: response to dietary excess; generation of precursor metabolites and energy; response to cAMP; ovarian follicle development; sexual reproduction; insulin secretion; defense response to bacterium; response to cold; glucose homeostasis; response to insulin stimulus