Rat Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) ELISA Kit | TNFRSF8 elisa kit

Rat Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) ELISA Kit

Cat. Num. AAA2088138

• Gene Names: TNFRSF8; CD30; Ki-1; D1S166E
• Reactivity: Rat
• Synonyms: Tumor Necrosis Factor Receptor Superfamily; Member 8 (TNFRSF8); Rat Tumor Necrosis Factor Receptor Superfamily; Member 8 (TNFRSF8) ELISA Kit; CD30; KI-1; CD30L receptor; Ki-1 antigen; Lymphocyte activation antigen CD30; TNFRSF8 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8).; No significant cross-reactivity or interference between Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) and analogues was observed.
• Sequence Length: 595

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.135ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFRSF8 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TNFRSF8 elisa kit

Signal transduction; CD & Adhesion molecule; Apoptosis; Tumor immunity; Reproductive science

NCBI and Uniprot Product Information

• NCBI GI #: 597709795
• NCBI GeneID: 943
• NCBI Accession #: NP_001234.3
• NCBI GenBank Nucleotide #: NM_001243.4
• UniProt Accession #: P28908; Q6P4D9; B1AN79; B9EGD9; D3YTD8
• Molecular Weight: 50,935 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 8 isoform 1
• NCBI Official Synonym Full Names: TNF receptor superfamily member 8
• NCBI Official Symbol: TNFRSF8
• NCBI Official Synonym Symbols: CD30; Ki-1; D1S166E
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 8
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 8
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF8
• UniProt Synonym Gene Names: CD30; D1S166E

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF8: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: integral to plasma membrane

Molecular Function: transmembrane receptor activity; tumor necrosis factor receptor activity

Biological Process: immune response; inflammatory response; multicellular organismal development; negative regulation of cell proliferation; positive regulation of apoptosis; positive regulation of TRAIL biosynthetic process; positive regulation of tumor necrosis factor biosynthetic process; response to lipopolysaccharide; signal transduction