Human CD30/TNFRSF8 PicoKine ELISA Kit | TNFRSF8 elisa kit

Human CD30/TNFRSF8 PicoKine ELISA Kit

Cat. Num. AAA177619

• Gene Names: TNFRSF8; CD30; Ki-1; D1S166E
• Reactivity: Human
• Synonyms: CD30/TNFRSF8 PicoKine; Human CD30/TNFRSF8 PicoKine ELISA Kit; Tumor necrosis factor receptor superfamily member 8; CD 30; CD153; CD30; CD30 antigen; CD30L receptor; Cytokine receptor CD30; D1S166E; KI 1; Ki 1 antigen; Ki-1 antigen; KI1; Lymphocyte activation antigen CD30; TNFRSF 8; TNFRSF8; TNR8_HUMAN; Tumor Necrosis Factor Receptor Superfamily Member 8; tumor necrosis factor receptor superfamily; member 8; TNFRSF8 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CD30
• Sequence Length: 595

• Assay Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human CD30 in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, F19-K379
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml human CD30 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each human CD30 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(ng/ml)|0.48|1.3|5.2|0.52|2.4|6.3; Standard deviation|0.02|0.06|0.29|0.03|0.14|0.4; CV(%)|3.3|4.5|5.6|4.9|5.8|6.2
• Sample Data: Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000; O.D.|0.091|0.145|0.231|0.379|0.547|1.009|1.503|2.241
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human CD30/TNFRSF8 PicoKine ELISA Kit standard curve )

Related Product Information for TNFRSF8 elisa kit

Principle of the assay: Human CD30 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD30 has been precoated onto 96-well plates. Standards(NSO, F19-K379) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD30 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD30 amount of sample captured in plate.

Background: CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.

NCBI and Uniprot Product Information

• NCBI GI #: 597709795
• NCBI GeneID: 943
• NCBI Accession #: NP_001234.3
• NCBI GenBank Nucleotide #: NM_001243.4
• UniProt Accession #: P28908; Q6P4D9; B1AN79; B9EGD9; D3YTD8
• Molecular Weight: 50,935 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 8 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily member 8
• NCBI Official Symbol: TNFRSF8
• NCBI Official Synonym Symbols: CD30; Ki-1; D1S166E
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 8
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 8
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF8
• UniProt Synonym Gene Names: CD30; D1S166E
• UniProt Entry Name: TNR8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF8: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: cytoplasm; integral to plasma membrane

Molecular Function: transmembrane receptor activity; tumor necrosis factor receptor activity

Biological Process: immune response; inflammatory response; multicellular organismal development; negative regulation of cell proliferation; positive regulation of apoptosis; positive regulation of TRAIL biosynthetic process; positive regulation of tumor necrosis factor biosynthetic process; response to lipopolysaccharide; signal transduction; tumor necrosis factor-mediated signaling pathway