Typical Testing Data/Standard Curve (for reference only)
Rat Transcription factor Sp1 ELISA Kit | Sp1 elisa kit
Rat Transcription factor Sp1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription factor Sp1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Transcription factor Sp1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Transcription factor Sp1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Transcription factor Sp1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
transcription factor that recognizes 5'-CCGCCC promoter sequence; plays a role in transcriptional regulation of many genes [RGD, Feb 2006]
Uniprot Description
SP1: a transcription factor of the Sp1 C2H2-type zinc-finger protein family. Phosphorylated and activated by MAPK. Dephosphorylation by PTEN inhibits DNA binding. Binds to p38 in the nucleus. Interacts with Huntingtin and TAFII130. Transcriptional activity of SP1 and TAFII130 disrupted in early Huntingtin's Disease.
Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor
Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; nucleus
Molecular Function: bHLH transcription factor binding; DNA binding; double-stranded DNA binding; histone acetyltransferase binding; histone deacetylase binding; metal ion binding; protein binding; protein C-terminus binding; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity; transcription factor binding
Biological Process: embryonic camera-type eye morphogenesis; embryonic placenta development; embryonic process involved in female pregnancy; embryonic skeletal development; enucleate erythrocyte differentiation; in utero embryonic development; liver development; lung development; megakaryocyte differentiation; mRNA transcription from RNA polymerase II promoter; ossification; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; rhythmic process; transcription from RNA polymerase II promoter; trophectodermal cell differentiation
Research Articles on Sp1
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Product Notes
The Rat Sp1 sp1 (Catalog #AAA2884856) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2884856 ELISA Kit recognizes Rat Sp1. It is sometimes possible for the material contained within the vial of "Transcription factor Sp1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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