Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Syndecan 4 (SDC4) Standard Curve.)
Rat Syndecan 4 (SDC4) ELISA Kit | SDC4 elisa kit
Rat Syndecan 4 (SDC4) ELISA Kit
Gene Names
Sdc4; RYUDOCA; RATRYUDOCA
Reactivity
Rat
Synonyms
Syndecan 4 (SDC4); Rat Syndecan 4 (SDC4) ELISA Kit; SDC4; MGC22217; SYND4; amphiglycan; ryudocan amphiglycan; ryudocan core protein; syndecan 4 (amphiglycan; ryudocan); syndecan proteoglycan 4; SDC4 elisa kit
Reactivity
Rat
Specificity
Rat Syndecan 4 (SDC4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SDC4. No significant cross-reactivity or interference between Rat SDC4 and analogues was observed.
Sequence Length
202
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Syndecan 4 (SDC4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Syndecan 4 (SDC4) Standard Curve.)
Related Product Information for SDC4 elisa kit
Description: This Rat Syndecan 4 (SDC4) ELISA Kit employs a two-site sandwich ELISA to quantitate SDC4 in samples. An antibody specific for SDC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDC4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Syndecan-4 is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. Syndecan4 is upregulated in osteoarthritis and inhibition of syndecan 4 reduces cartilage destruction in mouse models of OA. Syndecans, including SDC4, can function as in trans HIV receptors via binding of HIV-1 gp120 to the syndecan heparan sulfate chains. Flow cytometric analysis demonstrated SDC expression on endothelial cells. HIV bound to SDC on endothelial cell lines maintained its infectivity for at least 1 week, compared with less than 1 day for unbound virus.
Background: Syndecan-4 is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. Syndecan4 is upregulated in osteoarthritis and inhibition of syndecan 4 reduces cartilage destruction in mouse models of OA. Syndecans, including SDC4, can function as in trans HIV receptors via binding of HIV-1 gp120 to the syndecan heparan sulfate chains. Flow cytometric analysis demonstrated SDC expression on endothelial cells. HIV bound to SDC on endothelial cell lines maintained its infectivity for at least 1 week, compared with less than 1 day for unbound virus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
syndecan-4
NCBI Official Synonym Full Names
syndecan 4
NCBI Official Symbol
Sdc4
NCBI Official Synonym Symbols
RYUDOCA; RATRYUDOCA
NCBI Protein Information
syndecan-4
UniProt Protein Name
Syndecan-4
Protein Family
UniProt Gene Name
Sdc4
UniProt Synonym Gene Names
Synd4; SYND4
NCBI Description
regulates cell-matrix interactions; may bind phospholipids and play a role in phosphoinositide-mediated signaling [RGD, Feb 2006]
Uniprot Description
Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
Research Articles on SDC4
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Product Notes
The Rat SDC4 sdc4 (Catalog #AAA9711905) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711905 ELISA Kit recognizes Rat SDC4. It is sometimes possible for the material contained within the vial of "Syndecan 4 (SDC4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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