Human Syndecan 4 ELISA Kit | SDC4 elisa kit

Human Syndecan 4 ELISA Kit

Cat. Num. AAA8244668

• Gene Names: SDC4; SYND4
• Reactivity: Human
• Applications: ELISA
• Synonyms: Syndecan 4; Human Syndecan 4 ELISA Kit; Syndecan-4; SYND4; Amphiglycan; Ryudocan core protein; SDC4 elisa kit

• Reactivity: Human
• Specificity: The Human Syndecan 4 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Syndecan 4 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
• Sequence Length: 198

• Applicable Applications for SDC4 elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum.
• Sensitivity: 10 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for SDC4 elisa kit

Principle of the Assay||The Human Syndecan 4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Syndecan 4 in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Human Syndecan 4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Syndecan 4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Syndecan 4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Syndecan 4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What's more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.

NCBI and Uniprot Product Information

• NCBI GI #: 2851418
• NCBI GeneID: 6385
• UniProt Accession #: P31431; O00773; Q16833; Q53FN9; Q6FGN3
• Molecular Weight: 16,545 Da
• NCBI Official Full Name: Syndecan-4
• NCBI Official Synonym Full Names: syndecan 4
• NCBI Official Symbol: SDC4
• NCBI Official Synonym Symbols: SYND4
• NCBI Protein Information: syndecan-4
• UniProt Protein Name: Syndecan-4
• Protein Family: Syndecan
• UniProt Gene Name: SDC4
• UniProt Synonym Gene Names: SYND4
• UniProt Entry Name: SDC4_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]

Uniprot Description

syndecan-4: a heparan sulfate proteoglycan type I membrane protein that belongs to the syndecan proteoglycan family. Expressed as a homodimer. Promotes cell spreading in a beta(1) integrin-dependent fashion through PKC-alpha and Rho.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: cell surface; costamere; focal adhesion; Golgi lumen; integral to plasma membrane; lipid raft; lysosomal lumen; plasma membrane

Molecular Function: fibronectin binding; protein binding; protein kinase C binding

Biological Process: carbohydrate metabolic process; cell migration; chondroitin sulfate metabolic process; extracellular matrix organization and biogenesis; fat-soluble vitamin metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; inner ear receptor stereocilium organization and biogenesis; neural tube closure; phototransduction, visible light; positive regulation of focal adhesion formation; positive regulation of protein kinase activity; positive regulation of stress fiber formation; retinoid metabolic process; ureteric bud development; vitamin metabolic process; wound healing