Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Synaptotagmin-like protein 5 (SYTL5) Standard Curve.)
Rat Synaptotagmin-Like Protein 5 (SYTL5) ELISA Kit | SYTL5 elisa kit
Rat Synaptotagmin-Like Protein 5 (SYTL5) ELISA Kit
Reactivity
Rat
Synonyms
Synaptotagmin-Like Protein 5 (SYTL5); Rat Synaptotagmin-Like Protein 5 (SYTL5) ELISA Kit; SYTL5; slp5; OTTHUMP00000031916; exophilin 9; SYTL5 elisa kit
Reactivity
Rat
Specificity
Rat Synaptotagmin-like protein 5 (SYTL5) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SYTL5. No significant cross-reactivity or interference between Rat SYTL5 and analogues was observed.
Sequence Length
753
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Synaptotagmin-like protein 5 (SYTL5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Synaptotagmin-like protein 5 (SYTL5) Standard Curve.)
Related Product Information for SYTL5 elisa kit
Description: This Rat Synaptotagmin-like protein 5 (SYTL5) ELISA Kit employs a two-site sandwich ELISA to quantitate SYTL5 in samples. An antibody specific for SYTL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYTL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYTL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYTL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The protein encoded by SYTL5 belongs to the synaptotagmin-like (Slp) protein family, which contains a unique homology domain at the N-terminus, referred to as the Slp homology domain (SHD). The SHD functions as a binding site for Rab27A, which plays a role in protein transport. Expression of this gene is restricted to placenta and liver, suggesting that it might be involved in Rab27A-dependent membrane trafficking in specific tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Background: The protein encoded by SYTL5 belongs to the synaptotagmin-like (Slp) protein family, which contains a unique homology domain at the N-terminus, referred to as the Slp homology domain (SHD). The SHD functions as a binding site for Rab27A, which plays a role in protein transport. Expression of this gene is restricted to placenta and liver, suggesting that it might be involved in Rab27A-dependent membrane trafficking in specific tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
synaptotagmin-like protein 5
NCBI Official Synonym Full Names
synaptotagmin-like 5
NCBI Official Symbol
Sytl5
NCBI Protein Information
synaptotagmin-like protein 5
UniProt Protein Name
Synaptotagmin-like protein 5
Protein Family
UniProt Gene Name
Sytl5
UniProt Synonym Gene Names
Slp5
UniProt Entry Name
SYTL5_RAT
NCBI Description
human homolog binds specifically to the GTP-bound form of Rab27A, binding occurs via the Slp homology domain SHD; may play a role in membrane trafficking [RGD, Feb 2006]
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Product Notes
The Rat SYTL5 sytl5 (Catalog #AAA9711779) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711779 ELISA Kit recognizes Rat SYTL5. It is sometimes possible for the material contained within the vial of "Synaptotagmin-Like Protein 5 (SYTL5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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