Rat Sterol regulatory element-binding protein 1 (SREBP1) ELISA Kit | SREBP1 elisa kit

Rat Sterol regulatory element-binding protein 1 (SREBP1) ELISA Kit

Cat. Num. AAA1600312

• Gene Names: SREBF1; SREBP1; bHLHd1
• Reactivity: Rat
• Synonyms: Sterol regulatory element-binding protein 1 (SREBP1); Rat Sterol regulatory element-binding protein 1 (SREBP1) ELISA Kit; SREBP1 elisa kit

• Reactivity: Rat
• Sequence Length: 1123

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.3ng/ml - 90ng/ml
• Sensitivity: 0.16ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SREBP1 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Sterol Regulatory Element-binding Protein 1 (also known as SREBP1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat SREBP1 antibody. SREBP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SREBP1 Antibody is added and binds to SREBP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SREBP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat SREBP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 1007361599
• NCBI GeneID: 6720
• NCBI Accession #: NP_001308025.1
• NCBI GenBank Nucleotide #: NM_001321096.2
• Molecular Weight: 113,562 Da
• NCBI Official Full Name: sterol regulatory element-binding protein 1 isoform c
• NCBI Official Synonym Full Names: sterol regulatory element binding transcription factor 1
• NCBI Official Symbol: SREBF1
• NCBI Official Synonym Symbols: SREBP1; bHLHd1
• NCBI Protein Information: sterol regulatory element-binding protein 1
• UniProt Protein Name: Sterol regulatory element-binding protein 1
• UniProt Gene Name: SREBF1
• UniProt Synonym Gene Names: BHLHD1; SREBP1; SREBP-1; bHLHd1

NCBI Description

This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively. [provided by RefSeq, Nov 2017]

Uniprot Description

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').