Western Blot (WB)
(Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with PERP antibody at 1 μg/mL. )
Rabbit anti-Human PERP Polyclonal Antibody | anti-Perp antibody
PERP Antibody
Gene Names
Perp; THW; KCP1; PIGPC1; KRTCAP1; 1110017A08Rik
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
PERP Antibody is Antibody is DEAE purified.
Synonyms
PERP; Polyclonal Antibody; PERP Antibody; THW; KCP1; PIGPC1; KRTCAP1; 1110017A08Rik; Krtcap1; p53 apoptosis effector related to PMP-22; Keratinocyte-associated protein 1; KCP-1; TP53 apoptosis effector; anti-Perp antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
PERP Antibody is Antibody is DEAE purified.
Form/Format
Liquid
Sequence Length
193
Applicable Applications for anti-Perp antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
PERP antibody can be used for detection of PERP by Western blot at 1 mug/mL. A 21 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 mug/mL.
Conjugate
Unconjugated
Immunogen
PERP antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human PERP, which differ from the mouse sequence by three amino acids.
Buffer
PERP Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
PERP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with PERP antibody at 1 μg/mL. )
Western Blot (WB)
(Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with PERP antibody at 1 μg/mL. )
Immunocytochemistry (IHC)
(Immunocytochemistry of PERP in A431 cells with PERP antibody at 10 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of PERP in A431 cells with PERP antibody at 10 μg/mL.)
Related Product Information for anti-Perp antibody
PERP Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21 kDa
NCBI Official Full Name
p53 apoptosis effector related to PMP-22
NCBI Official Synonym Full Names
PERP, TP53 apoptosis effector
NCBI Official Symbol
Perp
NCBI Official Synonym Symbols
THW; KCP1; PIGPC1; KRTCAP1; 1110017A08Rik
NCBI Protein Information
p53 apoptosis effector related to PMP-22; KCP-1; p53 apoptosis-associated target; keratinocyte-associated protein 1; p53 apoptosis effector related to Pmp22
UniProt Protein Name
p53 apoptosis effector related to PMP-22
Protein Family
UniProt Gene Name
Perp
UniProt Synonym Gene Names
Krtcap1; KCP-1
UniProt Entry Name
PERP_MOUSE
Uniprot Description
PERP: Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway. Belongs to the TMEM47 family.
Protein type: Membrane protein, multi-pass; Mitochondrial; Cell adhesion; Membrane protein, integral
Cellular Component: Golgi apparatus; mitochondrion; membrane; integral to plasma membrane; integral to membrane; plasma membrane; cell junction
Biological Process: heterotypic cell-cell adhesion; apoptosis; positive regulation of proteolysis; cell adhesion
Research Articles on Perp
Similar Products
Product Notes
The Perp perp (Catalog #AAA151420) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PERP Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PERP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC). PERP antibody can be used for detection of PERP by Western blot at 1 mug/mL. A 21 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. Researchers should empirically determine the suitability of the Perp perp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PERP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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