Rabbit anti-Human FGD4 Polyclonal Antibody | anti-FGD4 antibody

FGD4 Antibody (N-term)

Cat. Num. AAA9204764

• Gene Names: FGD4; CMT4H; FRABP; ZFYVE6
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: FGD4; Polyclonal Antibody; FGD4 Antibody (N-term); FYVE; RhoGEF and PH domain-containing protein 4; Actin filament-binding protein frabin; FGD1-related F-actin-binding protein; Zinc finger FYVE domain-containing protein 6; FRABP; ZFYVE6; anti-FGD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This FGD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-186 amino acids from the N-terminal region of human FGD4.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.45 (varies by lot)
• Sequence Positions: 157-186
• Sequence Length: 188

• Applicable Applications for anti-FGD4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(FGD4 Antibody (N-term) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the FGD4 antibody detected the FGD4 protein (arrow).)

Related Product Information for anti-FGD4 antibody

This gene encodes a protein that is involved in the regulation of the actin cytoskeleton and cell shape. This protein contains an actin filament-binding domain, which together with its Dbl homology domain and one of its pleckstrin homology domains, can form microspikes. This protein can activate MAPK8 independently of the actin filament-binding domain, and it is also involved in the activation of CDC42 via the exchange of bound GDP for free GTP. The activation of CDC42 also enables this protein to play a role in mediating the cellular invasion of Cryptosporidium parvum, an intracellular parasite that infects the gastrointestinal tract. Mutations in this gene can cause Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the peripheral nervous system. [provided by RefSeq].

References

Rose, J. Phd, et al. Mol. Med. (2010) In press : Fabrizi, G.M., et al. Neurology 72(13):1160-1164(2009) Houlden, H., et al. Neurology 72(7):617-620(2009) Horlock, C., et al. Int. Arch. Allergy Immunol. 150(3):237-251(2009) Stendel, C., et al. Am. J. Hum. Genet. 81(1):158-164(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 198041928
• NCBI GeneID: 121512
• NCBI Accession #: NP_640334.2
• NCBI GenBank Nucleotide #: NM_139241.3
• UniProt Accession #: Q96M96; Q6ULS2; Q8TCP6
• Molecular Weight: 86626
• NCBI Official Full Name: FYVE, RhoGEF and PH domain-containing protein 4 isoform 3
• NCBI Official Synonym Full Names: FYVE, RhoGEF and PH domain containing 4
• NCBI Official Symbol: FGD4
• NCBI Official Synonym Symbols: CMT4H; FRABP; ZFYVE6
• NCBI Protein Information: FYVE, RhoGEF and PH domain-containing protein 4
• UniProt Protein Name: FYVE, RhoGEF and PH domain-containing protein 4
• Protein Family: FYVE, RhoGEF and PH domain-containing protein
• UniProt Gene Name: FGD4
• UniProt Synonym Gene Names: FRABP; ZFYVE6
• UniProt Entry Name: FGD4_HUMAN

NCBI Description

This gene encodes a protein that is involved in the regulation of the actin cytoskeleton and cell shape. This protein contains an actin filament-binding domain, which together with its Dbl homology domain and one of its pleckstrin homology domains, can form microspikes. This protein can activate MAPK8 independently of the actin filament-binding domain, and it is also involved in the activation of CDC42 via the exchange of bound GDP for free GTP. The activation of CDC42 also enables this protein to play a role in mediating the cellular invasion of Cryptosporidium parvum, an intracellular parasite that infects the gastrointestinal tract. Mutations in this gene can cause Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the peripheral nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]

Uniprot Description

FGD4: Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. Activates MAPK8. Defects in FGD4 are the cause of Charcot-Marie-Tooth disease type 4H (CMT4H); also known as Charcot-Marie- Tooth disease neuropathy type 4H. CMT4H is a recessive demyelinating form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot- Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 12p11.21

Cellular Component: ruffle; Golgi apparatus; lamellipodium; cytoplasm; cytosol; filopodium; actin cytoskeleton

Molecular Function: small GTPase binding; Rho guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; metal ion binding; actin binding

Biological Process: lamellipodium biogenesis; regulation of cell shape; regulation of small GTPase mediated signal transduction; filopodium formation; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; microspike biogenesis; cytoskeleton organization and biogenesis; actin cytoskeleton organization and biogenesis; positive regulation of GTPase activity

Disease: Charcot-marie-tooth Disease, Type 4h