Chicken Connexin 40 ELISA Kit | CX40 elisa kit

Chicken Connexin 40 ELISA Kit

Cat. Num. AAA740145

• Gene Names: GJA5; CX40; ATFB11
• Reactivity: Chicken
• Synonyms: Connexin 40; Chicken Connexin 40 ELISA Kit; CX40 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This CX40 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken CX40. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CX40 elisa kit

Principle of the Assay: CX40 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CX40. Standards or samples are then added to the microtiter plate wells and CX40 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CX40 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CX40 are added to each well to "sandwich" the CX40 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CX40 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX40 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CX40 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 5007068
• NCBI GeneID: 2702
• Molecular Weight: 40,380 Da
• NCBI Official Full Name: connexin 40
• NCBI Official Synonym Full Names: gap junction protein, alpha 5, 40kDa
• NCBI Official Symbol: GJA5
• NCBI Official Synonym Symbols: CX40; ATFB11
• NCBI Protein Information: gap junction alpha-5 protein; connexin 40; gap junction protein alpha 5 40kDa
• UniProt Protein Name: Gap junction alpha-5 protein
• UniProt Gene Name: GJA5
• UniProt Synonym Gene Names: Cx40
• UniProt Entry Name: CXA5_HUMAN

NCBI Description

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

GJA5: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJA5 are a cause of familial atrial standstill (FAS). Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm. Defects in GJA5 are the cause of familial atrial fibrillation type 11 (ATFB11). ATFB11 is a familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the connexin family. Alpha-type (group II) subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Motility/polarity/chemotaxis; Channel, misc.

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: connexon complex; cell projection; integral to plasma membrane; gap junction

Molecular Function: gap junction hemi-channel activity

Biological Process: negative regulation of glomerular filtration; gap junction assembly; positive regulation of vasodilation; protein oligomerization; positive regulation of vasoconstriction; embryonic heart tube development; artery morphogenesis; angiogenesis; negative regulation of blood pressure; transmembrane transport; skeletal development; potassium ion transport; embryonic limb morphogenesis

Disease: Atrial Standstill 1; Atrial Fibrillation, Familial, 11