Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Sphingosine-1-phosphate phosphatase 1 (SGPP1) Standard Curve.)
Rat Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) ELISA Kit | SGPP1 elisa kit
Rat Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) ELISA Kit
Gene Names
Sgpp1; Spp1
Reactivity
Rat
Synonyms
Sphingosine-1-Phosphate Phosphatase 1 (SGPP1); Rat Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) ELISA Kit; SGPP1; SPPase1; sphingosine-1-phosphatase; SGPP1 elisa kit
Reactivity
Rat
Specificity
Rat Sphingosine-1-phosphate phosphatase 1 (SGPP1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SGPP1. No significant cross-reactivity or interference between Rat SGPP1 and analogues was observed.
Sequence Length
430
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Sphingosine-1-phosphate phosphatase 1 (SGPP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Sphingosine-1-phosphate phosphatase 1 (SGPP1) Standard Curve.)
Related Product Information for SGPP1 elisa kit
Description: This Rat Sphingosine-1-phosphate phosphatase 1 (SGPP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SGPP1 in samples. An antibody specific for SGPP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SGPP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SGPP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SGPP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides. The deduced 430-amino acid mouse protein shares 17% amino acid identity with yeast Lbp1. The deduced 441-amino acid protein shares 76% amino acid identity with its mouse homolog. Transfection of HEK293 or Chinese hamster ovary (CHO) cells with SGPP1 resulted in marked increase in SGPP1 activity in membrane fractions. SGPP1 was highly specific toward long-chain sphingoid base phosphates and degraded S1P, dihydro-S1P, and phyto-S1P. SGPP1 activity was independent of any cation requirements, including Mg(2+), and was not inhibited by EDTA but was inhibited by Zn(2+).
Background: Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides. The deduced 430-amino acid mouse protein shares 17% amino acid identity with yeast Lbp1. The deduced 441-amino acid protein shares 76% amino acid identity with its mouse homolog. Transfection of HEK293 or Chinese hamster ovary (CHO) cells with SGPP1 resulted in marked increase in SGPP1 activity in membrane fractions. SGPP1 was highly specific toward long-chain sphingoid base phosphates and degraded S1P, dihydro-S1P, and phyto-S1P. SGPP1 activity was independent of any cation requirements, including Mg(2+), and was not inhibited by EDTA but was inhibited by Zn(2+).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Sphingosine-1-phosphate phosphatase 1
NCBI Official Synonym Full Names
sphingosine-1-phosphate phosphatase 1
NCBI Official Symbol
Sgpp1
NCBI Official Synonym Symbols
Spp1
NCBI Protein Information
sphingosine-1-phosphate phosphatase 1
UniProt Protein Name
Sphingosine-1-phosphate phosphatase 1
Protein Family
UniProt Gene Name
Sgpp1
UniProt Synonym Gene Names
SPPase1; Spp1
NCBI Description
mouse homolog is a phosphatase involved in the regulation of S1P activity [RGD, Feb 2006]
Uniprot Description
Has enzymatic activity against both sphingosine 1-phosphate (S1P) and dihydro-S1P. Regulates intracellular and extracellular S1P levels ().
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Product Notes
The Rat SGPP1 sgpp1 (Catalog #AAA9711884) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711884 ELISA Kit recognizes Rat SGPP1. It is sometimes possible for the material contained within the vial of "Sphingosine-1-Phosphate Phosphatase 1 (SGPP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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