Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Secreted phosphoprotein 24 (SPP2) Standard Curve.)
Rat Secreted Phosphoprotein 24 (SPP2) ELISA Kit | SPP24 elisa kit
Rat Secreted Phosphoprotein 24 (SPP2) ELISA Kit
Reactivity
Rat
Synonyms
Secreted Phosphoprotein 24 (SPP2); Rat Secreted Phosphoprotein 24 (SPP2) ELISA Kit; SPP2; OTTHUMP00000065382; secreted phosphoprotein 24; SPP24 elisa kit
Reactivity
Rat
Specificity
Rat Secreted phosphoprotein 24 (SPP2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SPP2. No significant cross-reactivity or interference between Rat SPP2 and analogues was observed.
Sequence Length
203
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Secreted phosphoprotein 24 (SPP2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Secreted phosphoprotein 24 (SPP2) Standard Curve.)
Related Product Information for SPP24 elisa kit
Description: This Rat Secreted phosphoprotein 24 (SPP2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPP2 in samples. An antibody specific for SPP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP2 catalyzes the degradation of S1P.The deduced 399-amino acid protein has a calculated molecular mass of 44.7 kD and shares 39.3% amino acid identity with SGPP1. SGPP2 has an N-terminal hydrophobic region, 3 conserved phosphatase-family motifs, and is predicted to be an integral membrane protein with as many as 9 membrane-spanning segments. Immunofluorescence microscopy showed a reticular staining pattern in the perinuclear and cytosolic regions of HEK293 cells, and SGPP2 colocalized with an endoplasmic reticulum antibody. Northern blot analysis of human tissues detected a 5.1-kb transcript with high expression in kidney and heart, followed by brain, colon, lung, and small intestine.
Background: Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP2 catalyzes the degradation of S1P.The deduced 399-amino acid protein has a calculated molecular mass of 44.7 kD and shares 39.3% amino acid identity with SGPP1. SGPP2 has an N-terminal hydrophobic region, 3 conserved phosphatase-family motifs, and is predicted to be an integral membrane protein with as many as 9 membrane-spanning segments. Immunofluorescence microscopy showed a reticular staining pattern in the perinuclear and cytosolic regions of HEK293 cells, and SGPP2 colocalized with an endoplasmic reticulum antibody. Northern blot analysis of human tissues detected a 5.1-kb transcript with high expression in kidney and heart, followed by brain, colon, lung, and small intestine.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
secreted phosphoprotein 24
NCBI Official Synonym Full Names
secreted phosphoprotein 2
NCBI Official Symbol
Spp2
NCBI Protein Information
secreted phosphoprotein 24
UniProt Protein Name
Secreted phosphoprotein 24
UniProt Gene Name
Spp2
UniProt Synonym Gene Names
Spp24; Spp-24
UniProt Entry Name
SPP24_RAT
Uniprot Description
SPP2: Could coordinate an aspect of bone turnover. Belongs to the SPP2 family.
Protein type: Inhibitor; Secreted, signal peptide; Secreted
Cellular Component: protein complex
Biological Process: protein complex assembly; bone remodeling
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Product Notes
The Rat SPP24 spp2 (Catalog #AAA9711733) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711733 ELISA Kit recognizes Rat SPP24. It is sometimes possible for the material contained within the vial of "Secreted Phosphoprotein 24 (SPP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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