MFAP4 recombinant protein

MFAP4, 22-255aa, Human, His-tag, Baculovirus

Cat. Num. AAA206483

• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: MFAP4; 22-255aa; Human; His-tag; Baculovirus; Microfibril-associated glycoprotein 4 isoform 2; MFAP4 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADLVSGIRGD ALERFCLQQP LDCDDIYAQG YQSDGVYLIY PSGPSVPVPV FCDMTTEGGK WTVFQKRFNG SVSFFRGWND YKLGFGRADG EYWLGLQNMH LLTLKQKYEL RVDLEDFENN TAYAKYADFS ISPNAVSAEE DGYTLFVAGF EDGGAGDSLS YHSGQKFSTF DRDQDLFVQN CAALSSGAFW FRSCHFANLN GFYLGGSHLS YANGINWAQW KGFYYSLKRT EMKIRRAHHH HHH
• Sequence Length: 255

• Applicable Applications for MFAP4 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for MFAP4 recombinant protein

MFAP4, also as known as microfibrillar-associated protein 4, belongs to Fibrinogen protein family and contains 1 fibrinogen C-terminal domain. This protein has similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell adhesion or intercellular interactions. Deletion of MFAP4 was found in 30 of 31 patients with Smith-Magenis syndrome (SMS), a clinically recognizable multiple congenital anomaly/mental retardation syndrome. Recombinant human MFAP4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for MFAP4 recombinant protein

Signal Transduction; Cytoskeleton

References

Lausen M. et al., (1999) J Biol Chem. 274:32234-32240.; Zhao Z. et al., (1995) Mol. Genet. 4: 589-597.

NCBI and Uniprot Product Information

• NCBI GI #: 23111005
• NCBI GeneID: 4239
• NCBI Accession #: NP_002395
• NCBI GenBank Nucleotide #: NM_002404.2
• UniProt Accession #: P55083; Q6P680; A8KAJ1; A8MVM2; B4E317
• Molecular Weight: 27.5kDa (243aa) 28-40KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: microfibril-associated glycoprotein 4 isoform 2
• NCBI Official Synonym Full Names: microfibril associated protein 4
• NCBI Official Symbol: MFAP4
• NCBI Protein Information: microfibril-associated glycoprotein 4
• UniProt Protein Name: Microfibril-associated glycoprotein 4
• Protein Family: Microfibril-associated glycoprotein
• UniProt Gene Name: MFAP4

NCBI Description

This gene encodes a protein with similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell adhesion or intercellular interactions. The gene is located within the Smith-Magenis syndrome region. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

Could be involved in calcium-dependent cell adhesion or intercellular interactions. May contribute to the elastic fiber assembly and/or maintenance (PubMed:26601954).