Rat Serine/threonine-protein kinase STK11 ELISA Kit | STK11 elisa kit

Rat Serine/threonine-protein kinase STK11 ELISA Kit

Cat. Num. AAA761571

• Gene Names: STK11; LKB1
• Reactivity: Rat
• Synonyms: Serine/threonine-protein kinase STK11; Rat Serine/threonine-protein kinase STK11 ELISA Kit; STK11/PJS/EC 2.7.11.1/LKB1 serine/threonine kinase 11 (Peutz-Jeghers syndrome)/PJS/PJS polarization-related protein LKB1/Renal carcinoma antigen NY-REN-19/serine/threonine kinase 11/serine/threonine-protein kinase 11/Serine/threonine-protein kinase LKB1; STK11 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of STK11. No significant cross-reactivity or interference between STK11 and analogues was observed.
• Sequence Length: 440

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level STK11 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level STK11 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STK11 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- STK11 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- STK11 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the STK11 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of STK11 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 113951717
• NCBI GeneID: 420105
• NCBI Accession #: NP_001039298.1
• NCBI GenBank Nucleotide #: NM_001045833.1
• Molecular Weight: 49,793 Da
• NCBI Official Full Name: serine/threonine-protein kinase STK11
• NCBI Official Synonym Full Names: serine/threonine kinase 11
• NCBI Official Symbol: STK11
• NCBI Official Synonym Symbols: LKB1
• NCBI Protein Information: serine/threonine-protein kinase STK11
• UniProt Protein Name: Serine/threonine-protein kinase STK11
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: STK11
• UniProt Synonym Gene Names: LKB1
• UniProt Entry Name: STK11_CHICK

Uniprot Description

Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, leading to promote their activity ().