Human STK31 (Serine/threonine-protein kinase 31) ELISA Kit | STK31 elisa kit

Human STK31 (Serine/threonine-protein kinase 31) ELISA Kit

Cat. Num. AAA7607197

• Gene Names: STK31; TDRD8; SGK396
• Reactivity: Human
• Synonyms: STK31 (Serine/threonine-protein kinase 31); Human STK31 (Serine/threonine-protein kinase 31) ELISA Kit; STK31 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Stat3. No significant cross-reactivity or interference between Stat3 and analogues was observed.
• Sequence Length: 1019

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stat3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stat3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STK31 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Stat3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Stat3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Stat3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Stat3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 14602443
• NCBI GeneID: 56164
• NCBI Accession #: NP_113602.2
• NCBI GenBank Nucleotide #: NM_031414.5
• UniProt Accession #: Q9BXU1; Q6PCD3; Q9BXH8; B4DZ06; B7WPP5; C9J4F9
• Molecular Weight: 113,260 Da
• NCBI Official Full Name: serine/threonine-protein kinase 31 isoform a
• NCBI Official Synonym Full Names: serine/threonine kinase 31
• NCBI Official Symbol: STK31
• NCBI Official Synonym Symbols: TDRD8; SGK396
• NCBI Protein Information: serine/threonine-protein kinase 31
• UniProt Protein Name: Serine/threonine-protein kinase 31
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: STK31
• UniProt Synonym Gene Names: SGK396; SgK396

NCBI Description

This gene is similar to a mouse gene that encodes a putative protein kinase with a tudor domain, and shows testis-specific expression. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CautionSer-854 is present instead of the conserved Asp which is expected to be an active site residue.