Human DNA Repair Protein RAD50 (RAD50) ELISA Kit | RAD50 elisa kit

Human DNA Repair Protein RAD50 (RAD50) ELISA Kit

Cat. Num. AAA2706812

• Gene Names: RAD50; NBSLD; RAD502; hRad50
• Reactivity: Human
• Synonyms: DNA Repair Protein RAD50 (RAD50); Human DNA Repair Protein RAD50 (RAD50) ELISA Kit; RAD50 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of DNA Repair Protein RAD50 (RAD50).; No significant cross-reactivity or interference between DNA Repair Protein RAD50 (RAD50) and analogues was observed.
• Sequence Length: 1,173

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DNA Repair Protein RAD50 (RAD50) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DNA Repair Protein RAD50 (RAD50) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAD50 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Repair Protein RAD50 (RAD50). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Repair Protein RAD50 (RAD50). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Repair Protein RAD50 (RAD50), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNA Repair Protein RAD50 (RAD50) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19924129
• NCBI GeneID: 10111
• NCBI Accession #: NP_005723.2
• NCBI GenBank Nucleotide #: NM_005732.3
• UniProt Accession #: Q92878; O43254; Q6GMT7; Q6P5X3; Q9UP86; B9EGF5
• Molecular Weight: 138,432 Da
• NCBI Official Full Name: DNA repair protein RAD50
• NCBI Official Synonym Full Names: RAD50 double strand break repair protein
• NCBI Official Symbol: RAD50
• NCBI Official Synonym Symbols: NBSLD; RAD502; hRad50
• NCBI Protein Information: DNA repair protein RAD50
• UniProt Protein Name: DNA repair protein RAD50
• Protein Family: DNA repair protein
• UniProt Gene Name: RAD50
• UniProt Synonym Gene Names: hRAD50

NCBI Description

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]

Uniprot Description

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:11741547, PubMed:9590181, PubMed:9705271, PubMed:9651580). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).