Typical Testing Data/Standard Curve (for reference only)
Rat Serine/threonine-protein kinase B-raf (BRAF) ELISA Kit | BRAF elisa kit
Rat Serine/threonine-protein kinase B-raf (BRAF) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: BRAF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BRAF antibody and an BRAF-HRP conjugate. The assay sample and buffer are incubated together with BRAF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BRAF concentration since BRAF from samples and BRAF-HRP conjugate compete for the anti-BRAF antibody binding site. Since the number of sites is limited, as more sites are occupied by BRAF from the sample, fewer sites are left to bind BRAF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BRAF concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
BRAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib.
Protein type: Oncoprotein; Protein kinase, TKL; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); TKL group; RAF family
Cellular Component: neuron projection; mitochondrion; plasma membrane; nucleus
Molecular Function: protein serine/threonine kinase activity; identical protein binding; receptor signaling protein activity; calcium ion binding; ATP binding
Biological Process: positive T cell selection; CD4-positive, alpha beta T cell differentiation; myeloid progenitor cell differentiation; somatic stem cell maintenance; positive regulation of stress fiber formation; visual learning; negative regulation of neuron apoptosis; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; regulation of cell proliferation
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Product Notes
The Rat BRAF braf (Catalog #AAA7217205) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7217205 ELISA Kit recognizes Rat BRAF. It is sometimes possible for the material contained within the vial of "Serine/threonine-protein kinase B-raf (BRAF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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