Rat Forkhead Box Protein O1 ELISA Kit | FOXO1 elisa kit

Rat Forkhead Box Protein O1 ELISA Kit

Cat. Num. AAA749342

• Gene Names: FOXO1; FKH1; FKHR; FOXO1A
• Reactivity: Rat
• Synonyms: Forkhead Box Protein O1; Rat Forkhead Box Protein O1 ELISA Kit; FOXO1 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/ml
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FOXO1 elisa kit

Intended Uses||This FOXO1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FOXO1. This ELISA kit for research use only!

Principle of the Assay: FOXO1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-FOXO1 antibody and an FOXO1-HRP conjugate. The assay sample and buffer are incubated together with FOXO1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FOXO1 concentration since FOXO1 from samples and FOXO1-HRP conjugate compete for the anti-FOXO1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FOXO1 from the sample, fewer sites are left to bind FOXO1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FOXO1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FOXO1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 9257222
• NCBI GeneID: 2308
• NCBI Accession #: NP_002006.2
• NCBI GenBank Nucleotide #: NM_002015.3
• Molecular Weight: 69,662 Da
• NCBI Official Full Name: forkhead box protein O1
• NCBI Official Synonym Full Names: forkhead box O1
• NCBI Official Symbol: FOXO1
• NCBI Official Synonym Symbols: FKH1; FKHR; FOXO1A
• NCBI Protein Information: forkhead box protein O1; forkhead box protein O1A; forkhead, Drosophila, homolog of, in rhabdomyosarcoma
• UniProt Protein Name: Forkhead box protein O1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXO1
• UniProt Synonym Gene Names: FKHR; FOXO1A
• UniProt Entry Name: FOXO1_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain.

Protein type: Oncoprotein; DNA-binding; Transcription factor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 13q14.1

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding; chromatin binding

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; thermoregulation; positive regulation of gluconeogenesis; positive regulation of autophagy; epidermal growth factor receptor signaling pathway; blood vessel development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cellular response to starvation; negative regulation of stress-activated MAPK cascade; negative regulation of fat cell differentiation; endocrine pancreas development; protein amino acid acetylation; cellular response to insulin stimulus; insulin receptor signaling pathway; positive regulation of protein catabolic process; cell glucose homeostasis; innate immune response; autophagy; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus; negative regulation of apoptosis

Disease: Rhabdomyosarcoma 2