Mouse Serine/threonine-protein kinase 4 (STK4) ELISA Kit | STK4 elisa kit

Mouse Serine/threonine-protein kinase 4 (STK4) ELISA Kit

Cat. Num. AAA7200584

• Reactivity: Mouse
• Synonyms: Serine/threonine-protein kinase 4 (STK4); Mouse Serine/threonine-protein kinase 4 (STK4) ELISA Kit; STK4 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Competitive (Quantitative)
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STK4 elisa kit

Intended Uses: This STK4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse STK4. This ELISA kit for research use only!

Principle of the Assay: STK4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-STK4 antibody and an STK4-HRP conjugate. The assay sample and buffer are incubated together with STK4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STK4 concentration since STK4 from samples and STK4-HRP conjugate compete for the anti-STK4 antibody binding site. Since the number of sites is limited, as more sites are occupied by STK4 from the sample, fewer sites are left to bind STK4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STK4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for STK4 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 683473792
• UniProt Accession #: Q5ZJK4
• Molecular Weight: 55,337 Da
• NCBI Official Full Name: Serine/threonine-protein kinase 4, partial
• UniProt Protein Name: Serine/threonine-protein kinase 4
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: STK4
• UniProt Synonym Gene Names: RCJMB04_17i1; MST1/N; MST1/C
• UniProt Entry Name: STK4_CHICK

Uniprot Description

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation ().