Rabbit anti-Human GOLPH3 Polyclonal Antibody | anti-GOLPH3 antibody

GOLPH3, NT (Golgi Phosphoprotein 3, Coat Protein GPP34, FLJ90675, GPP34, GOPP1, Mitochondrial DNA Absence Factor, MIDAS) (Biotin)

Cat. Num. AAA6478706

• Gene Names: GOLPH3; GOPP1; GPP34; MIDAS; Vps74
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: GOLPH3; Polyclonal Antibody; NT (Golgi Phosphoprotein 3; Coat Protein GPP34; FLJ90675; GPP34; GOPP1; Mitochondrial DNA Absence Factor; MIDAS) (Biotin); Golgi Phosphoprotein 3 (Coat-protein); anti-GOLPH3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GOLPH3.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GOLPH3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human GOLPH3 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GOLPH3 antibody (N-term) in Hela cell line lysates (35ug/lane). GOLPH3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with GOLPH3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using GOLPH3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GOLPH3 antibody

Antibodies; Golgi Proteins

References

1. Ota T., Suzuki Y., Nishikawa T.Nat. Genet. 36:40-45(2004). 2. Bell A.W., Ward M.A.J. Biol. Chem. 276:5152-5165(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 11545859
• NCBI GeneID: 64083
• NCBI Accession #: NP_071413
• NCBI GenBank Nucleotide #: NM_022130
• UniProt Accession #: Q9H4A6; Q9UIW5
• Molecular Weight: 33,811 Da
• NCBI Official Full Name: Golgi phosphoprotein 3
• NCBI Official Synonym Full Names: golgi phosphoprotein 3 (coat-protein)
• NCBI Official Symbol: GOLPH3
• NCBI Official Synonym Symbols: GOPP1; GPP34; MIDAS; Vps74
• NCBI Protein Information: Golgi phosphoprotein 3; coat protein GPP34; coat-protein; golgi peripheral membrane protein 1, 34 kDa; golgi protein; golgi-associated protein; mitochondrial DNA absence factor
• UniProt Protein Name: Golgi phosphoprotein 3
• Protein Family: Golgi phosphoprotein
• UniProt Gene Name: GOLPH3
• UniProt Synonym Gene Names: GPP34; MIDAS
• UniProt Entry Name: GOLP3_HUMAN

Uniprot Description

GOLPH3: Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. Belongs to the GOLPH3/VPS74 family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 5p13.3

Cellular Component: nucleoplasm; Golgi apparatus; Golgi cisterna; mitochondrion; plasma membrane; mitochondrial intermembrane space; trans-Golgi network; cytosol; endosome

Molecular Function: protein binding; enzyme binding

Biological Process: lamellipodium biogenesis; cell proliferation; cell migration; positive regulation of protein secretion; glycoprotein biosynthetic process; Golgi to plasma membrane protein transport; protein secretion; gene expression; Golgi vesicle budding; leukocyte tethering or rolling; protein retention in Golgi; Golgi organization and biogenesis; positive regulation of TOR signaling pathway